Restriction map for
pFN21AE6235
(Linear) (Six-base) MAPSORT of: KIEE6235.seq Check: 7383 from: 1 to: 747
KIEE6235.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AflIII A'CryG_T
Cuts at: 0 238 747
Size: 238 509
AleI CACnn'nnGTG
Cuts at: 0 88 747
Size: 88 659
AlwNI CAG_nnn'CTG
Cuts at: 0 521 747
Size: 521 226
BanI G'GyrC_C
Cuts at: 0 133 196 747
Size: 133 63 551
Fragments arranged by size:
551 133 63
BanII G_rGCy'C
Cuts at: 0 308 747
Size: 308 439
BbsI GAAGACnn'nnnn_
Cuts at: 0 66 747
Size: 66 681
BlpI GC'TnA_GC
Cuts at: 0 302 747
Size: 302 445
Bme1580I G_kGCm'C
Cuts at: 0 136 747
Size: 136 611
BmrI ACTGGGnnnn_n'
Cuts at: 0 95 672 747
Size: 95 577 75
Fragments arranged by size:
577 95 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 517 671 747
Size: 495 22 154 76
Fragments arranged by size:
495 154 76 22
Bpu10I CC'TnA_GC
Cuts at: 0 442 747
Size: 442 305
BsaI GGTCTCn'nnnn_
Cuts at: 0 13 747
Size: 13 734
BsaBI GATnn'nnATC
Cuts at: 0 542 747
Size: 542 205
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 156 186 747
Size: 156 30 561
Fragments arranged by size:
561 156 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 24 43 423 747
Size: 24 19 380 324
Fragments arranged by size:
380 324 24 19
BseYI C'CCAG_C
Cuts at: 0 514 747
Size: 514 233
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 478 747
Size: 451 27 269
Fragments arranged by size:
451 269 27
BsiHKAI G_wGCw'C
Cuts at: 0 185 308 481 747
Size: 185 123 173 266
Fragments arranged by size:
266 185 173 123
BsmI GAATG_Cn'
Cuts at: 0 670 747
Size: 670 77
Bsp1286I G_dGCh'C
Cuts at: 0 136 185 308 481 747
Size: 136 49 123 173 266
Fragments arranged by size:
266 173 136 123 49
BspHI T'CATG_A
Cuts at: 0 339 747
Size: 339 408
BsrDI GCAATG_nn'
Cuts at: 0 405 747
Size: 405 342
BstXI CCAn_nnnn'nTGG
Cuts at: 0 685 747
Size: 685 62
BstYI r'GATC_y
Cuts at: 0 552 747
Size: 552 195
Bsu36I CC'TnA_GG
Cuts at: 0 361 747
Size: 361 386
BtgI C'CryG_G
Cuts at: 0 253 747
Size: 253 494
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 630 747
Size: 630 117
BtsI GCAGTG_nn'
Cuts at: 0 425 747
Size: 425 322
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 189 224 747
Size: 189 35 523
Fragments arranged by size:
523 189 35
EaeI y'GGCC_r
Cuts at: 0 572 725 747
Size: 572 153 22
Fragments arranged by size:
572 153 22
EcoICRI GAG'CTC
Cuts at: 0 306 747
Size: 306 441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 517 671 747
Size: 495 22 154 76
Fragments arranged by size:
495 154 76 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 360 747
Size: 360 387
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 372 747
Size: 372 375
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 24 56 747
Size: 24 32 691
Fragments arranged by size:
691 32 24
HincII GTy'rAC
Cuts at: 0 588 747
Size: 588 159
HpaI GTT'AAC
Cuts at: 0 588 747
Size: 588 159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 747
Size: 87 660
MslI CAynn'nnrTG
Cuts at: 0 88 747
Size: 88 659
MspA1I CmG'CkG
Cuts at: 0 518 747
Size: 518 229
NcoI C'CATG_G
Cuts at: 0 253 747
Size: 253 494
NspI r_CATG'y
Cuts at: 0 242 693 747
Size: 242 451 54
Fragments arranged by size:
451 242 54
PciI A'CATG_T
Cuts at: 0 238 747
Size: 238 509
PfoI T'CCnGG_A
Cuts at: 0 494 747
Size: 494 253
PvuII CAG'CTG
Cuts at: 0 518 747
Size: 518 229
SacI G_AGCT'C
Cuts at: 0 308 747
Size: 308 439
SpeI A'CTAG_T
Cuts at: 0 616 747
Size: 616 131
SphI G_CATG'C
Cuts at: 0 693 747
Size: 693 54
StyI C'CwwG_G
Cuts at: 0 253 747
Size: 253 494
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 156 188 747
Size: 156 32 559
Fragments arranged by size:
559 156 32
Enzymes that do cut:
AflIII AleI AlwNI BanI BanII BbsI BlpI Bme1580I
BmrI BpmI Bpu10I BsaI BsaBI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI EaeI EcoICRI Eco57MI
EcoNI FalI Hin4I HincII HpaI MmeI MslI MspA1I
NcoI NspI PciI PfoI PvuII SacI SpeI SphI
StyI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BmgBI BmtI
BplI BpuEI BsaAI BsaHI BsaWI BsiEI BsiWI BsmBI
BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I ClaI DraI DraIII DrdI EagI
EarI EciI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII HindIII KasI KpnI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SrfI SspI StuI
SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI