Restriction map for
pFN21AE6232
(Linear) (Six-base) MAPSORT of: KIEE6232.seq Check: 514 from: 1 to: 900
KIEE6232.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 900
Size: 169 731
AlwNI CAG_nnn'CTG
Cuts at: 0 763 900
Size: 763 137
ApaI G_GGCC'C
Cuts at: 0 57 900
Size: 57 843
ApoI r'AATT_y
Cuts at: 0 874 900
Size: 874 26
AvaI C'yCGr_G
Cuts at: 0 437 900
Size: 437 463
BanII G_rGCy'C
Cuts at: 0 57 728 900
Size: 57 671 172
Fragments arranged by size:
671 172 57
BlpI GC'TnA_GC
Cuts at: 0 299 900
Size: 299 601
Bme1580I G_kGCm'C
Cuts at: 0 57 900
Size: 57 843
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 135 364 785 900
Size: 93 42 229 421 115
Fragments arranged by size:
421 229 115 93 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 96 683 900
Size: 96 587 217
Fragments arranged by size:
587 217 96
BsaAI yAC'GTr
Cuts at: 0 883 900
Size: 883 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 90 190 447 450 900
Size: 90 100 257 3 450
Fragments arranged by size:
450 257 100 90 3
BseYI C'CCAG_C
Cuts at: 0 24 45 900
Size: 24 21 855
Fragments arranged by size:
855 24 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 486 900
Size: 486 414
BsiHKAI G_wGCw'C
Cuts at: 0 757 900
Size: 757 143
Bsp1286I G_dGCh'C
Cuts at: 0 57 728 757 900
Size: 57 671 29 143
Fragments arranged by size:
671 143 57 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 228 900
Size: 228 672
BstXI CCAn_nnnn'nTGG
Cuts at: 0 294 900
Size: 294 606
Bsu36I CC'TnA_GG
Cuts at: 0 85 900
Size: 85 815
BtgI C'CryG_G
Cuts at: 0 413 900
Size: 413 487
BtsI GCAGTG_nn'
Cuts at: 0 498 900
Size: 498 402
DraIII CAC_nnn'GTG
Cuts at: 0 895 900
Size: 895 5
DrdI GACnn_nn'nnGTC
Cuts at: 0 514 900
Size: 514 386
EaeI y'GGCC_r
Cuts at: 0 59 704 900
Size: 59 645 196
Fragments arranged by size:
645 196 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 135 169 364 785 900
Size: 93 42 34 195 421 115
Fragments arranged by size:
421 195 115 93 42 34
EcoO109I rG'GnC_Cy
Cuts at: 0 54 192 476 769 900
Size: 54 138 284 293 131
Fragments arranged by size:
293 284 138 131 54
HaeII r_GCGC'y
Cuts at: 0 68 900
Size: 68 832
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 268 300 506 538 900
Size: 268 32 206 32 362
Fragments arranged by size:
362 268 206 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 260 900
Size: 229 31 640
Fragments arranged by size:
640 229 31
MscI TGG'CCA
Cuts at: 0 706 900
Size: 706 194
MslI CAynn'nnrTG
Cuts at: 0 208 613 900
Size: 208 405 287
Fragments arranged by size:
405 287 208
MspA1I CmG'CkG
Cuts at: 0 24 900
Size: 24 876
NcoI C'CATG_G
Cuts at: 0 413 900
Size: 413 487
PasI CC'CwG_GG
Cuts at: 0 103 900
Size: 103 797
PfoI T'CCnGG_A
Cuts at: 0 428 900
Size: 428 472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 527 559 900
Size: 527 32 341
Fragments arranged by size:
527 341 32
PpuMI rG'GwC_Cy
Cuts at: 0 192 769 900
Size: 192 577 131
Fragments arranged by size:
577 192 131
PspOMI G'GGCC_C
Cuts at: 0 53 900
Size: 53 847
PstI C_TGCA'G
Cuts at: 0 144 818 849 900
Size: 144 674 31 51
Fragments arranged by size:
674 144 51 31
PvuII CAG'CTG
Cuts at: 0 24 900
Size: 24 876
SexAI A'CCwGG_T
Cuts at: 0 829 891 900
Size: 829 62 9
Fragments arranged by size:
829 62 9
SfcI C'TryA_G
Cuts at: 0 140 814 845 900
Size: 140 674 31 55
Fragments arranged by size:
674 140 55 31
SmaI CCC'GGG
Cuts at: 0 439 900
Size: 439 461
StyI C'CwwG_G
Cuts at: 0 386 413 900
Size: 386 27 487
Fragments arranged by size:
487 386 27
TatI w'GTAC_w
Cuts at: 0 818 900
Size: 818 82
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 879 900
Size: 879 21
Tth111I GACn'n_nGTC
Cuts at: 0 424 900
Size: 424 476
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 110 900
Size: 110 790
XmaI C'CCGG_G
Cuts at: 0 437 900
Size: 437 463
Enzymes that do cut:
AcuI AlwNI ApaI ApoI AvaI BanII BlpI Bme1580I
BpmI BsaI BsaAI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BstXI Bsu36I BtgI BtsI DraIII DrdI EaeI
Eco57MI EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I
NcoI PasI PfoI PpiI PpuMI PspOMI PstI PvuII
SexAI SfcI SmaI StyI TatI TstI Tth111I XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgZI ClaI CspCI DraI EagI EarI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII XbaI
XhoI XmnI ZraI