Restriction map for
pFN21AE6230
(Linear) (Six-base) MAPSORT of: KIEE6230.seq Check: 2498 from: 1 to: 900
KIEE6230.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AccI GT'mk_AC
Cuts at: 0 239 900
Size: 239 661
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 838 900
Size: 326 512 62
Fragments arranged by size:
512 326 62
AfeI AGC'GCT
Cuts at: 0 172 900
Size: 172 728
AflIII A'CryG_T
Cuts at: 0 234 422 900
Size: 234 188 478
Fragments arranged by size:
478 234 188
AlwNI CAG_nnn'CTG
Cuts at: 0 595 900
Size: 595 305
ApoI r'AATT_y
Cuts at: 0 215 484 900
Size: 215 269 416
Fragments arranged by size:
416 269 215
AvaI C'yCGr_G
Cuts at: 0 129 178 900
Size: 129 49 722
Fragments arranged by size:
722 129 49
BamHI G'GATC_C
Cuts at: 0 628 765 900
Size: 628 137 135
Fragments arranged by size:
628 137 135
BanI G'GyrC_C
Cuts at: 0 137 160 900
Size: 137 23 740
Fragments arranged by size:
740 137 23
BanII G_rGCy'C
Cuts at: 0 66 900
Size: 66 834
BbeI G_GCGC'C
Cuts at: 0 141 900
Size: 141 759
BbsI GAAGACnn'nnnn_
Cuts at: 0 317 900
Size: 317 583
BciVI GTATCCnnnnn_n'
Cuts at: 0 97 900
Size: 97 803
BglII A'GATC_T
Cuts at: 0 387 900
Size: 387 513
Bme1580I G_kGCm'C
Cuts at: 0 163 900
Size: 163 737
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 85 900
Size: 85 815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 546 900
Size: 454 92 354
Fragments arranged by size:
454 354 92
BsaI GGTCTCn'nnnn_
Cuts at: 0 175 900
Size: 175 725
BsaHI Gr'CG_yC
Cuts at: 0 80 138 900
Size: 80 58 762
Fragments arranged by size:
762 80 58
BsiHKAI G_wGCw'C
Cuts at: 0 116 900
Size: 116 784
Bsp1286I G_dGCh'C
Cuts at: 0 66 116 163 900
Size: 66 50 47 737
Fragments arranged by size:
737 66 50 47
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 572 900
Size: 572 328
BsrBI CCG'CTC
Cuts at: 0 152 900
Size: 152 748
BsrFI r'CCGG_y
Cuts at: 0 47 900
Size: 47 853
BstAPI GCAn_nnn'nTGC
Cuts at: 0 595 900
Size: 595 305
BstYI r'GATC_y
Cuts at: 0 387 628 765 900
Size: 387 241 137 135
Fragments arranged by size:
387 241 137 135
Bsu36I CC'TnA_GG
Cuts at: 0 872 900
Size: 872 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 210 900
Size: 210 690
BtsI GCAGTG_nn'
Cuts at: 0 151 900
Size: 151 749
ClaI AT'CG_AT
Cuts at: 0 790 900
Size: 790 110
DraIII CAC_nnn'GTG
Cuts at: 0 476 900
Size: 476 424
EaeI y'GGCC_r
Cuts at: 0 2 900
Size: 2 898
EarI CTCTTCn'nnn_
Cuts at: 0 732 900
Size: 732 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 454 546 838 900
Size: 326 128 92 292 62
Fragments arranged by size:
326 292 128 92 62
EcoRI G'AATT_C
Cuts at: 0 484 900
Size: 484 416
HaeII r_GCGC'y
Cuts at: 0 141 174 900
Size: 141 33 726
Fragments arranged by size:
726 141 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 772 804 865 897 900
Size: 772 32 61 32 3
Fragments arranged by size:
772 61 32 32 3
KasI G'GCGC_C
Cuts at: 0 137 900
Size: 137 763
MscI TGG'CCA
Cuts at: 0 4 900
Size: 4 896
MspA1I CmG'CkG
Cuts at: 0 417 900
Size: 417 483
NaeI GCC'GGC
Cuts at: 0 49 900
Size: 49 851
NarI GG'CG_CC
Cuts at: 0 138 900
Size: 138 762
NgoMIV G'CCGG_C
Cuts at: 0 47 900
Size: 47 853
NspI r_CATG'y
Cuts at: 0 238 426 900
Size: 238 188 474
Fragments arranged by size:
474 238 188
PciI A'CATG_T
Cuts at: 0 234 422 900
Size: 234 188 478
Fragments arranged by size:
478 234 188
PsiI TTA'TAA
Cuts at: 0 796 900
Size: 796 104
PstI C_TGCA'G
Cuts at: 0 541 900
Size: 541 359
RsrII CG'GwC_CG
Cuts at: 0 154 900
Size: 154 746
SfcI C'TryA_G
Cuts at: 0 537 834 900
Size: 537 297 66
Fragments arranged by size:
537 297 66
SfoI GGC'GCC
Cuts at: 0 139 900
Size: 139 761
SspI AAT'ATT
Cuts at: 0 810 900
Size: 810 90
StuI AGG'CCT
Cuts at: 0 718 900
Size: 718 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 711 900
Size: 711 189
TatI w'GTAC_w
Cuts at: 0 206 900
Size: 206 694
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 73 780 900
Size: 73 707 120
Fragments arranged by size:
707 120 73
Enzymes that do cut:
AccI AcuI AfeI AflIII AlwNI ApoI AvaI BamHI
BanI BanII BbeI BbsI BciVI BglII Bme1580I BplI
BpmI BsaI BsaHI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BstAPI BstYI Bsu36I BtgZI BtsI ClaI DraIII EaeI
EarI Eco57MI EcoRI HaeII Hin4I KasI MscI MspA1I
NaeI NarI NgoMIV NspI PciI PsiI PstI RsrII
SfcI SfoI SspI StuI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbvCI BcgI BclI BfrBI BglI BlpI BmgBI
BmrI BmtI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I BtgI CspCI DraI DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MslI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StyI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI