Restriction map for
pFN21AE6228
(Linear) (Six-base) MAPSORT of: KIEE6228.seq Check: 4254 from: 1 to: 780
KIEE6228.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 276 697 780
Size: 276 421 83
Fragments arranged by size:
421 276 83
AccI GT'mk_AC
Cuts at: 0 277 506 780
Size: 277 229 274
Fragments arranged by size:
277 274 229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 317 780
Size: 238 79 463
Fragments arranged by size:
463 238 79
AflIII A'CryG_T
Cuts at: 0 38 780
Size: 38 742
AleI CACnn'nnGTG
Cuts at: 0 707 780
Size: 707 73
ApaI G_GGCC'C
Cuts at: 0 252 780
Size: 252 528
AvaI C'yCGr_G
Cuts at: 0 773 780
Size: 773 7
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 225 258 780
Size: 225 33 522
Fragments arranged by size:
522 225 33
BamHI G'GATC_C
Cuts at: 0 84 780
Size: 84 696
BanII G_rGCy'C
Cuts at: 0 252 380 563 626 780
Size: 252 128 183 63 154
Fragments arranged by size:
252 183 154 128 63
BbsI GAAGACnn'nnnn_
Cuts at: 0 694 780
Size: 694 86
Bme1580I G_kGCm'C
Cuts at: 0 252 780
Size: 252 528
BmrI ACTGGGnnnn_n'
Cuts at: 0 177 780
Size: 177 603
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 490 780
Size: 226 264 290
Fragments arranged by size:
290 264 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 780
Size: 523 257
BsaI GGTCTCn'nnnn_
Cuts at: 0 55 299 780
Size: 55 244 481
Fragments arranged by size:
481 244 55
BsaAI yAC'GTr
Cuts at: 0 555 780
Size: 555 225
BsaWI w'CCGG_w
Cuts at: 0 87 780
Size: 87 693
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 664 780
Size: 664 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 715 780
Size: 112 603 65
Fragments arranged by size:
603 112 65
BsiEI CG_ry'CG
Cuts at: 0 240 780
Size: 240 540
BsiHKAI G_wGCw'C
Cuts at: 0 152 780
Size: 152 628
BsmBI CGTCTCn'nnnn_
Cuts at: 0 706 780
Size: 706 74
Bsp1286I G_dGCh'C
Cuts at: 0 152 252 380 563 626 780
Size: 152 100 128 183 63 154
Fragments arranged by size:
183 154 152 128 100 63
BspHI T'CATG_A
Cuts at: 0 671 780
Size: 671 109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 276 697 780
Size: 276 421 83
Fragments arranged by size:
421 276 83
BsrDI GCAATG_nn'
Cuts at: 0 573 780
Size: 573 207
BsrFI r'CCGG_y
Cuts at: 0 482 780
Size: 482 298
BssSI C'ACGA_G
Cuts at: 0 517 780
Size: 517 263
BstEII G'GTnAC_C
Cuts at: 0 723 780
Size: 723 57
BstYI r'GATC_y
Cuts at: 0 84 780
Size: 84 696
BtsI GCAGTG_nn'
Cuts at: 0 280 780
Size: 280 500
DraIII CAC_nnn'GTG
Cuts at: 0 132 780
Size: 132 648
DrdI GACnn_nn'nnGTC
Cuts at: 0 502 780
Size: 502 278
EaeI y'GGCC_r
Cuts at: 0 513 780
Size: 513 267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 238 317 490 780
Size: 226 12 79 173 290
Fragments arranged by size:
290 226 173 79 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 646 780
Size: 646 134
EcoO109I rG'GnC_Cy
Cuts at: 0 248 249 780
Size: 248 1 531
Fragments arranged by size:
531 248 1
FspI TGC'GCA
Cuts at: 0 745 780
Size: 745 35
MscI TGG'CCA
Cuts at: 0 515 780
Size: 515 265
MslI CAynn'nnrTG
Cuts at: 0 676 707 780
Size: 676 31 73
Fragments arranged by size:
676 73 31
NaeI GCC'GGC
Cuts at: 0 484 780
Size: 484 296
NgoMIV G'CCGG_C
Cuts at: 0 482 780
Size: 482 298
NspI r_CATG'y
Cuts at: 0 42 780
Size: 42 738
PciI A'CATG_T
Cuts at: 0 38 780
Size: 38 742
PfoI T'CCnGG_A
Cuts at: 0 578 780
Size: 578 202
PmlI CAC'GTG
Cuts at: 0 555 780
Size: 555 225
PspOMI G'GGCC_C
Cuts at: 0 248 780
Size: 248 532
PstI C_TGCA'G
Cuts at: 0 287 780
Size: 287 493
SexAI A'CCwGG_T
Cuts at: 0 44 780
Size: 44 736
SfcI C'TryA_G
Cuts at: 0 283 780
Size: 283 497
SmaI CCC'GGG
Cuts at: 0 775 780
Size: 775 5
SmlI C'TyrA_G
Cuts at: 0 538 780
Size: 538 242
StyI C'CwwG_G
Cuts at: 0 65 599 780
Size: 65 534 181
Fragments arranged by size:
534 181 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 43 172 780
Size: 43 129 608
Fragments arranged by size:
608 129 43
TatI w'GTAC_w
Cuts at: 0 521 608 780
Size: 521 87 172
Fragments arranged by size:
521 172 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 206 238 439 471 780
Size: 206 32 201 32 309
Fragments arranged by size:
309 206 201 32 32
XmaI C'CCGG_G
Cuts at: 0 773 780
Size: 773 7
Enzymes that do cut:
AarI AccI AcuI AflIII AleI ApaI AvaI BaeI
BamHI BanII BbsI Bme1580I BmrI BpmI BpuEI BsaI
BsaAI BsaWI BseRI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrFI BssSI BstEII BstYI BtsI
DraIII DrdI EaeI Eco57MI EcoNI EcoO109I FspI MscI
MslI NaeI NgoMIV NspI PciI PfoI PmlI PspOMI
PstI SexAI SfcI SmaI SmlI StyI TaqII TatI
TstI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BplI Bpu10I BsaBI BsaHI
BsaXI BseYI BsiWI BsmI BspEI BsrBI BsrGI BssHII
BstAPI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI EagI EarI EciI EcoICRI EcoRI EcoRV
FalI FseI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MspA1I NarI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI
XmnI ZraI