(Linear) (Six-base) MAPSORT of: KIEE6217.seq  Check: 2929  from: 1  to: 792



KIEE6217.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

AatII G_ACGT'C

Cuts at:      0     356     792 
   Size:        356     436
 
AccI GT'mk_AC

Cuts at:      0     386     792 
   Size:        386     406
 
AflIII A'CryG_T

Cuts at:      0     515     792 
   Size:        515     277
 
AlwNI CAG_nnn'CTG

Cuts at:      0     713     792 
   Size:        713      79
 
ApoI r'AATT_y

Cuts at:      0     694     792 
   Size:        694      98
 
BamHI G'GATC_C

Cuts at:      0     419     557     792 
   Size:        419     138     235

  Fragments arranged by size: 

                 419     235     138
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     436     519     792 
   Size:        436      83     273

  Fragments arranged by size: 

                 436     273      83
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     271     792 
   Size:        271     521
 
BglI GCCn_nnn'nGGC

Cuts at:      0     102     792 
   Size:        102     690
 
BglII A'GATC_T

Cuts at:      0     506     792 
   Size:        506     286
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      80     277     721     792 
   Size:         80     197     444      71

  Fragments arranged by size: 

                 444     197      80      71
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     739     792 
   Size:        739      53
 
BsaHI Gr'CG_yC

Cuts at:      0     275     353     451     792 
   Size:        275      78      98     341

  Fragments arranged by size: 

                 341     275      98      78
 
BsaWI w'CCGG_w

Cuts at:      0     593     792 
   Size:        593     199
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     176     206     792 
   Size:        176      30     586

  Fragments arranged by size: 

                 586     176      30
 
BspEI T'CCGG_A

Cuts at:      0     593     792 
   Size:        593     199
 
BsrFI r'CCGG_y

Cuts at:      0      96     666     792 
   Size:         96     570     126

  Fragments arranged by size: 

                 570     126      96
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     271     792 
   Size:        271     521
 
BstYI r'GATC_y

Cuts at:      0      38     419     506     557     792 
   Size:         38     381      87      51     235

  Fragments arranged by size: 

                 381     235      87      51      38
 
Bsu36I CC'TnA_GG

Cuts at:      0     298     792 
   Size:        298     494
 
BtgI C'CryG_G

Cuts at:      0     145     792 
   Size:        145     647
 
BtsI GCAGTG_nn'

Cuts at:      0     353     792 
   Size:        353     439
 
EcoO109I rG'GnC_Cy

Cuts at:      0      47     792 
   Size:         47     745
 
HaeII r_GCGC'y

Cuts at:      0      58     116     792 
   Size:         58      58     676

  Fragments arranged by size: 

                 676      58      58
 
HincII GTy'rAC

Cuts at:      0     336     792 
   Size:        336     456
 
HindIII A'AGCT_T

Cuts at:      0     757     792 
   Size:        757      35
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     243     792 
   Size:        243     549
 
MslI CAynn'nnrTG

Cuts at:      0     269     283     311     792 
   Size:        269      14      28     481

  Fragments arranged by size: 

                 481     269      28      14
 
NspI r_CATG'y

Cuts at:      0     519     792 
   Size:        519     273
 
PasI CC'CwG_GG

Cuts at:      0     713     792 
   Size:        713      79
 
PciI A'CATG_T

Cuts at:      0     515     792 
   Size:        515     277
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      26     792 
   Size:         26     766
 
SmlI C'TyrA_G

Cuts at:      0     754     792 
   Size:        754      38
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     176     208     792 
   Size:        176      32     584

  Fragments arranged by size: 

                 584     176      32
 
ZraI GAC'GTC

Cuts at:      0     354     792 
   Size:        354     438
 

 Enzymes that do cut:

    AatII     AccI   AflIII    AlwNI     ApoI    BamHI     BbsI    BciVI
     BglI    BglII     BmrI    BpuEI    BsaHI    BsaWI    BsaXI    BspEI
    BsrFI    BstXI    BstYI   Bsu36I     BtgI     BtsI EcoO109I    HaeII
   HincII  HindIII     MmeI     MslI     NspI     PasI     PciI    PflMI
     SmlI     TstI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI     BcgI
     BclI    BfrBI     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaBI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I     HpaI     KasI     KpnI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI