(Linear) (Six-base) MAPSORT of: KIEE6216.seq  Check: 5933  from: 1  to: 579



KIEE6216.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     579 
   Size:        259     320
 
AflIII A'CryG_T

Cuts at:      0     347     579 
   Size:        347     232
 
AleI CACnn'nnGTG

Cuts at:      0     332     579 
   Size:        332     247
 
AlwNI CAG_nnn'CTG

Cuts at:      0       9     579 
   Size:          9     570
 
ApoI r'AATT_y

Cuts at:      0     451     579 
   Size:        451     128
 
BanII G_rGCy'C

Cuts at:      0     371     383     579 
   Size:        371      12     196

  Fragments arranged by size: 

                 371     196      12
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     537     571     579 
   Size:        537      34       8

  Fragments arranged by size: 

                 537      34       8
 
BclI T'GATC_A

Cuts at:      0     140     579 
   Size:        140     439
 
BglI GCCn_nnn'nGGC

Cuts at:      0     483     579 
   Size:        483      96
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     371     473     579 
   Size:        371     102     106

  Fragments arranged by size: 

                 371     106     102
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     251     579 
   Size:        251     328
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     490     579 
   Size:        178     312      89

  Fragments arranged by size: 

                 312     178      89
 
Bpu10I CC'TnA_GC

Cuts at:      0     520     579 
   Size:        520      59
 
BsaBI GATnn'nnATC

Cuts at:      0     286     579 
   Size:        286     293
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     224     579 
   Size:        224     355
 
BseYI C'CCAG_C

Cuts at:      0      86     579 
   Size:         86     493
 
Bsp1286I G_dGCh'C

Cuts at:      0     371     383     579 
   Size:        371      12     196

  Fragments arranged by size: 

                 371     196      12
 
BstYI r'GATC_y

Cuts at:      0     287     579 
   Size:        287     292
 
BtsI GCAGTG_nn'

Cuts at:      0     436     534     579 
   Size:        436      98      45

  Fragments arranged by size: 

                 436      98      45
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     172     579 
   Size:        172     407
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     259     490     579 
   Size:        178      81     231      89

  Fragments arranged by size: 

                 231     178      89      81
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      13     579 
   Size:         13     566
 
EcoO109I rG'GnC_Cy

Cuts at:      0     129     579 
   Size:        129     450
 
EcoRI G'AATT_C

Cuts at:      0     451     579 
   Size:        451     128
 
FspI TGC'GCA

Cuts at:      0      62     579 
   Size:         62     517
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       3      35     579 
   Size:          3      32     544

  Fragments arranged by size: 

                 544      32       3
 
MslI CAynn'nnrTG

Cuts at:      0     332     579 
   Size:        332     247
 
MspA1I CmG'CkG

Cuts at:      0     314     579 
   Size:        314     265
 
NspI r_CATG'y

Cuts at:      0     351     579 
   Size:        351     228
 
PasI CC'CwG_GG

Cuts at:      0      36     579 
   Size:         36     543
 
PciI A'CATG_T

Cuts at:      0     347     579 
   Size:        347     232
 
PfoI T'CCnGG_A

Cuts at:      0     180     579 
   Size:        180     399
 
PpuMI rG'GwC_Cy

Cuts at:      0     129     579 
   Size:        129     450
 
PvuII CAG'CTG

Cuts at:      0     314     579 
   Size:        314     265
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    AlwNI     ApoI    BanII     BcgI     BclI
     BglI     BmrI     BplI     BpmI   Bpu10I    BsaBI    BseRI    BseYI
 Bsp1286I    BstYI     BtsI     DrdI  Eco57MI    EcoNI EcoO109I    EcoRI
     FspI    Hin4I     MslI   MspA1I     NspI     PasI     PciI     PfoI
    PpuMI    PvuII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
    BciVI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmtI    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     EaeI     EagI     EarI     EciI  EcoICRI    EcoRV     FalI     FseI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI