Restriction map for
pFN21AE6215
(Linear) (Six-base) MAPSORT of: KIEE6215.seq Check: 4126 from: 1 to: 795
KIEE6215.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 179 260 795
Size: 37 142 81 535
Fragments arranged by size:
535 142 81 37
AfeI AGC'GCT
Cuts at: 0 461 795
Size: 461 334
BamHI G'GATC_C
Cuts at: 0 48 795
Size: 48 747
BanII G_rGCy'C
Cuts at: 0 736 795
Size: 736 59
BbvCI CC'TCA_GC
Cuts at: 0 402 795
Size: 402 393
BciVI GTATCCnnnnn_n'
Cuts at: 0 330 795
Size: 330 465
BmrI ACTGGGnnnn_n'
Cuts at: 0 125 795
Size: 125 670
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 795
Size: 760 35
Bpu10I CC'TnA_GC
Cuts at: 0 402 795
Size: 402 393
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 598 795
Size: 448 150 197
Fragments arranged by size:
448 197 150
BsaI GGTCTCn'nnnn_
Cuts at: 0 260 795
Size: 260 535
BseYI C'CCAG_C
Cuts at: 0 554 795
Size: 554 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 705 795
Size: 705 90
BsmI GAATG_Cn'
Cuts at: 0 279 795
Size: 279 516
Bsp1286I G_dGCh'C
Cuts at: 0 736 795
Size: 736 59
BssHII G'CGCG_C
Cuts at: 0 689 795
Size: 689 106
BstXI CCAn_nnnn'nTGG
Cuts at: 0 198 784 795
Size: 198 586 11
Fragments arranged by size:
586 198 11
BstYI r'GATC_y
Cuts at: 0 48 795
Size: 48 747
BtgI C'CryG_G
Cuts at: 0 482 783 795
Size: 482 301 12
Fragments arranged by size:
482 301 12
BtsI GCAGTG_nn'
Cuts at: 0 203 406 722 795
Size: 203 203 316 73
Fragments arranged by size:
316 203 203 73
ClaI AT'CG_AT
Cuts at: 0 647 795
Size: 647 148
EaeI y'GGCC_r
Cuts at: 0 42 695 752 795
Size: 42 653 57 43
Fragments arranged by size:
653 57 43 42
EarI CTCTTCn'nnn_
Cuts at: 0 131 419 795
Size: 131 288 376
Fragments arranged by size:
376 288 131
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 179 260 760 795
Size: 37 142 81 500 35
Fragments arranged by size:
500 142 81 37 35
EcoO109I rG'GnC_Cy
Cuts at: 0 705 795
Size: 705 90
HaeII r_GCGC'y
Cuts at: 0 463 795
Size: 463 332
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 795
Size: 106 32 657
Fragments arranged by size:
657 106 32
HindIII A'AGCT_T
Cuts at: 0 145 370 795
Size: 145 225 425
Fragments arranged by size:
425 225 145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 795
Size: 276 519
MscI TGG'CCA
Cuts at: 0 44 754 795
Size: 44 710 41
Fragments arranged by size:
710 44 41
MslI CAynn'nnrTG
Cuts at: 0 496 795
Size: 496 299
NcoI C'CATG_G
Cuts at: 0 482 783 795
Size: 482 301 12
Fragments arranged by size:
482 301 12
PasI CC'CwG_GG
Cuts at: 0 702 795
Size: 702 93
PstI C_TGCA'G
Cuts at: 0 729 795
Size: 729 66
SfcI C'TryA_G
Cuts at: 0 725 795
Size: 725 70
SmlI C'TyrA_G
Cuts at: 0 463 613 795
Size: 463 150 182
Fragments arranged by size:
463 182 150
StyI C'CwwG_G
Cuts at: 0 482 528 783 795
Size: 482 46 255 12
Fragments arranged by size:
482 255 46 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 533 795
Size: 533 262
Enzymes that do cut:
AcuI AfeI BamHI BanII BbvCI BciVI BmrI BpmI
Bpu10I BpuEI BsaI BseYI BsgI BsmI Bsp1286I BssHII
BstXI BstYI BtgI BtsI ClaI EaeI EarI Eco57MI
EcoO109I HaeII Hin4I HindIII MmeI MscI MslI NcoI
PasI PstI SfcI SmlI StyI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI
BbsI BcgI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HpaI KasI KpnI MfeI MluI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI