(Linear) (Six-base) MAPSORT of: KIEE6208.seq  Check: 9601  from: 1  to: 579



KIEE6208.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:27  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     472     579 
   Size:        472     107
 
ApaLI G'TGCA_C

Cuts at:      0     346     579 
   Size:        346     233
 
ApoI r'AATT_y

Cuts at:      0      77     579 
   Size:         77     502
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      76     110     579 
   Size:         76      34     469

  Fragments arranged by size: 

                 469      76      34
 
Bme1580I G_kGCm'C

Cuts at:      0     350     579 
   Size:        350     229
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     564     579 
   Size:        564      15
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     460     579 
   Size:        343     117     119

  Fragments arranged by size: 

                 343     119     117
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     579 
   Size:        103     476
 
BsiHKAI G_wGCw'C

Cuts at:      0     350     579 
   Size:        350     229
 
Bsp1286I G_dGCh'C

Cuts at:      0     350     579 
   Size:        350     229
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     515     579 
   Size:        515      64
 
BsrFI r'CCGG_y

Cuts at:      0     194     579 
   Size:        194     385
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     472     579 
   Size:        472     107
 
BstBI TT'CG_AA

Cuts at:      0     302     579 
   Size:        302     277
 
BtgI C'CryG_G

Cuts at:      0      30      40     579 
   Size:         30      10     539

  Fragments arranged by size: 

                 539      30      10
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     460     579 
   Size:        343     117     119

  Fragments arranged by size: 

                 343     119     117
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     268     361     579 
   Size:        268      93     218

  Fragments arranged by size: 

                 268     218      93
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      93     125     411     443     579 
   Size:         93      32     286      32     136

  Fragments arranged by size: 

                 286     136      93      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     368     579 
   Size:        368     211
 
NcoI C'CATG_G

Cuts at:      0      30     579 
   Size:         30     549
 
NdeI CA'TA_TG

Cuts at:      0     335     579 
   Size:        335     244
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     366     579 
   Size:        366     213
 
PfoI T'CCnGG_A

Cuts at:      0     359     579 
   Size:        359     220
 
SmlI C'TyrA_G

Cuts at:      0     118     579 
   Size:        118     461
 
StyI C'CwwG_G

Cuts at:      0      30     579 
   Size:         30     549
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     364     579 
   Size:        364     215
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      32      64     579 
   Size:         32      32     515

  Fragments arranged by size: 

                 515      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0      77     579 
   Size:         77     502
 

 Enzymes that do cut:

    AlwNI    ApaLI     ApoI     BcgI Bme1580I     BmrI     BpmI    BpuEI
  BsiHKAI Bsp1286I    BspMI    BsrFI   BstAPI    BstBI     BtgI  Eco57MI
    EcoNI    Hin4I     MmeI     NcoI     NdeI    PflMI     PfoI     SmlI
     StyI    TaqII     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI