(Linear) (Six-base) MAPSORT of: KIEE6206.seq  Check: 9304  from: 1  to: 594



KIEE6206.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:27  ..

AflIII A'CryG_T

Cuts at:      0     276     594 
   Size:        276     318
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     556     594 
   Size:        556      38
 
ApoI r'AATT_y

Cuts at:      0     501     594 
   Size:        501      93
 
AvaI C'yCGr_G

Cuts at:      0     367     594 
   Size:        367     227
 
BanII G_rGCy'C

Cuts at:      0     256     368     594 
   Size:        256     112     226

  Fragments arranged by size: 

                 256     226     112
 
BglI GCCn_nnn'nGGC

Cuts at:      0     372     594 
   Size:        372     222
 
BlpI GC'TnA_GC

Cuts at:      0     362     594 
   Size:        362     232
 
Bme1580I G_kGCm'C

Cuts at:      0     273     594 
   Size:        273     321
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     210     594 
   Size:        210     384
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     270     501     594 
   Size:        270     231      93

  Fragments arranged by size: 

                 270     231      93
 
Bpu10I CC'TnA_GC

Cuts at:      0      62     594 
   Size:         62     532
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      70     594 
   Size:         70     524
 
BsaAI yAC'GTr

Cuts at:      0      93     168     594 
   Size:         93      75     426

  Fragments arranged by size: 

                 426      93      75
 
BsaWI w'CCGG_w

Cuts at:      0      20     594 
   Size:         20     574
 
BseYI C'CCAG_C

Cuts at:      0      56     594 
   Size:         56     538
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370     594 
   Size:        370     224
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     517     563     594 
   Size:        517      46      31

  Fragments arranged by size: 

                 517      46      31
 
Bsp1286I G_dGCh'C

Cuts at:      0     256     273     368     594 
   Size:        256      17      95     226

  Fragments arranged by size: 

                 256     226      95      17
 
BspHI T'CATG_A

Cuts at:      0     413     594 
   Size:        413     181
 
BssHII G'CGCG_C

Cuts at:      0     331     594 
   Size:        331     263
 
BstYI r'GATC_y

Cuts at:      0     320     594 
   Size:        320     274
 
EaeI y'GGCC_r

Cuts at:      0     216     281     594 
   Size:        216      65     313

  Fragments arranged by size: 

                 313     216      65
 
EarI CTCTTCn'nnn_

Cuts at:      0      94     184     594 
   Size:         94      90     410

  Fragments arranged by size: 

                 410      94      90
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     270     501     594 
   Size:        270     231      93

  Fragments arranged by size: 

                 270     231      93
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     550     594 
   Size:        550      44
 
EcoO109I rG'GnC_Cy

Cuts at:      0     204     594 
   Size:        204     390
 
MspA1I CmG'CkG

Cuts at:      0      56     221     594 
   Size:         56     165     373

  Fragments arranged by size: 

                 373     165      56
 
NspI r_CATG'y

Cuts at:      0     280     594 
   Size:        280     314
 
PciI A'CATG_T

Cuts at:      0     276     594 
   Size:        276     318
 
PfoI T'CCnGG_A

Cuts at:      0     247     594 
   Size:        247     347
 
PmlI CAC'GTG

Cuts at:      0     168     594 
   Size:        168     426
 
PpuMI rG'GwC_Cy

Cuts at:      0     204     594 
   Size:        204     390
 
SapI GCTCTTCn'nnn_

Cuts at:      0     184     594 
   Size:        184     410
 
SexAI A'CCwGG_T

Cuts at:      0     235     594 
   Size:        235     359
 
SnaBI TAC'GTA

Cuts at:      0      93     594 
   Size:         93     501
 

 Enzymes that do cut:

   AflIII     AlfI     ApoI     AvaI    BanII     BglI     BlpI Bme1580I
     BmrI     BpmI   Bpu10I     BsaI    BsaAI    BsaWI    BseYI     BsgI
    BsmBI Bsp1286I    BspHI   BssHII    BstYI     EaeI     EarI  Eco57MI
    EcoNI EcoO109I   MspA1I     NspI     PciI     PfoI     PmlI    PpuMI
     SapI    SexAI    SnaBI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BmtI
     BplI    BpuEI    BsaBI    BsaHI    BsaXI    BseRI    BsiEI  BsiHKAI
    BsiWI     BsmI    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI