(Linear) (Six-base) MAPSORT of: KIEE6205.seq  Check: 1478  from: 1  to: 537



KIEE6205.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:27  ..

ApoI r'AATT_y

Cuts at:      0      69     481     537 
   Size:         69     412      56

  Fragments arranged by size: 

                 412      69      56
 
BglII A'GATC_T

Cuts at:      0     192     537 
   Size:        192     345
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     214     537 
   Size:        214     323
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     230     260     537 
   Size:        230      30     277

  Fragments arranged by size: 

                 277     230      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     203     537 
   Size:        203     334
 
BseYI C'CCAG_C

Cuts at:      0     507     537 
   Size:        507      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     158     537 
   Size:        158     379
 
Bsp1286I G_dGCh'C

Cuts at:      0     158     537 
   Size:        158     379
 
BspHI T'CATG_A

Cuts at:      0     303     464     537 
   Size:        303     161      73

  Fragments arranged by size: 

                 303     161      73
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     183     537 
   Size:        183     354
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     234     537 
   Size:        234     303
 
BstYI r'GATC_y

Cuts at:      0     192     222     537 
   Size:        192      30     315

  Fragments arranged by size: 

                 315     192      30
 
EarI CTCTTCn'nnn_

Cuts at:      0     261     537 
   Size:        261     276
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      48     537 
   Size:         48     489
 
MslI CAynn'nnrTG

Cuts at:      0     232     537 
   Size:        232     305
 
SfcI C'TryA_G

Cuts at:      0     278     537 
   Size:        278     259
 
StuI AGG'CCT

Cuts at:      0     371     537 
   Size:        371     166
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     380     537 
   Size:        380     157
 
XbaI T'CTAG_A

Cuts at:      0       9     537 
   Size:          9     528
 

 Enzymes that do cut:

     ApoI    BglII     BplI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I
    BspHI    BspMI    BstXI    BstYI     EarI     EciI     MslI     SfcI
     StuI    TaqII     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI
     ZraI