Restriction map for
pFN21AE6203
(Linear) (Six-base) MAPSORT of: KIEE6203.seq Check: 6807 from: 1 to: 669
KIEE6203.orf
With 182 enzymes: SgfI *
May 20, 2014 13:33 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 669
Size: 555 114
AvaI C'yCGr_G
Cuts at: 0 348 620 669
Size: 348 272 49
Fragments arranged by size:
348 272 49
BanI G'GyrC_C
Cuts at: 0 271 322 391 415 669
Size: 271 51 69 24 254
Fragments arranged by size:
271 254 69 51 24
BanII G_rGCy'C
Cuts at: 0 337 641 669
Size: 337 304 28
Fragments arranged by size:
337 304 28
BbeI G_GCGC'C
Cuts at: 0 275 669
Size: 275 394
BbsI GAAGACnn'nnnn_
Cuts at: 0 99 426 561 669
Size: 99 327 135 108
Fragments arranged by size:
327 135 108 99
BciVI GTATCCnnnnn_n'
Cuts at: 0 369 669
Size: 369 300
BglII A'GATC_T
Cuts at: 0 368 669
Size: 368 301
Bme1580I G_kGCm'C
Cuts at: 0 418 669
Size: 418 251
BmrI ACTGGGnnnn_n'
Cuts at: 0 369 669
Size: 369 300
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 669
Size: 655 14
BsaAI yAC'GTr
Cuts at: 0 216 669
Size: 216 453
BsaHI Gr'CG_yC
Cuts at: 0 272 527 669
Size: 272 255 142
Fragments arranged by size:
272 255 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 223 253 669
Size: 223 30 416
Fragments arranged by size:
416 223 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 669
Size: 244 425
BseYI C'CCAG_C
Cuts at: 0 87 148 669
Size: 87 61 521
Fragments arranged by size:
521 87 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 271 669
Size: 187 84 398
Fragments arranged by size:
398 187 84
BsiEI CG_ry'CG
Cuts at: 0 522 669
Size: 522 147
BsiHKAI G_wGCw'C
Cuts at: 0 641 669
Size: 641 28
BsmI GAATG_Cn'
Cuts at: 0 258 669
Size: 258 411
BsmBI CGTCTCn'nnnn_
Cuts at: 0 442 669
Size: 442 227
Bsp1286I G_dGCh'C
Cuts at: 0 337 418 641 669
Size: 337 81 223 28
Fragments arranged by size:
337 223 81 28
BsrFI r'CCGG_y
Cuts at: 0 17 669
Size: 17 652
BssSI C'ACGA_G
Cuts at: 0 160 669
Size: 160 509
BstXI CCAn_nnnn'nTGG
Cuts at: 0 503 669
Size: 503 166
BstYI r'GATC_y
Cuts at: 0 368 669
Size: 368 301
BtgI C'CryG_G
Cuts at: 0 11 339 669
Size: 11 328 330
Fragments arranged by size:
330 328 11
BtsI GCAGTG_nn'
Cuts at: 0 10 244 669
Size: 10 234 425
Fragments arranged by size:
425 234 10
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 464 499 669
Size: 464 35 170
Fragments arranged by size:
464 170 35
EaeI y'GGCC_r
Cuts at: 0 342 519 557 657 669
Size: 342 177 38 100 12
Fragments arranged by size:
342 177 100 38 12
EagI C'GGCC_G
Cuts at: 0 519 669
Size: 519 150
EarI CTCTTCn'nnn_
Cuts at: 0 113 322 601 611 669
Size: 113 209 279 10 58
Fragments arranged by size:
279 209 113 58 10
EcoICRI GAG'CTC
Cuts at: 0 639 669
Size: 639 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 669
Size: 555 114
EcoO109I rG'GnC_Cy
Cuts at: 0 624 663 669
Size: 624 39 6
Fragments arranged by size:
624 39 6
HaeII r_GCGC'y
Cuts at: 0 275 357 669
Size: 275 82 312
Fragments arranged by size:
312 275 82
KasI G'GCGC_C
Cuts at: 0 271 669
Size: 271 398
MscI TGG'CCA
Cuts at: 0 659 669
Size: 659 10
MslI CAynn'nnrTG
Cuts at: 0 505 669
Size: 505 164
MspA1I CmG'CkG
Cuts at: 0 58 341 481 669
Size: 58 283 140 188
Fragments arranged by size:
283 188 140 58
NaeI GCC'GGC
Cuts at: 0 19 669
Size: 19 650
NarI GG'CG_CC
Cuts at: 0 272 669
Size: 272 397
NgoMIV G'CCGG_C
Cuts at: 0 17 669
Size: 17 652
PasI CC'CwG_GG
Cuts at: 0 442 669
Size: 442 227
PflMI CCAn_nnn'nTGG
Cuts at: 0 165 669
Size: 165 504
PmlI CAC'GTG
Cuts at: 0 216 669
Size: 216 453
PpuMI rG'GwC_Cy
Cuts at: 0 663 669
Size: 663 6
PshAI GACnn'nnGTC
Cuts at: 0 70 669
Size: 70 599
PstI C_TGCA'G
Cuts at: 0 239 669
Size: 239 430
PvuII CAG'CTG
Cuts at: 0 481 669
Size: 481 188
SacI G_AGCT'C
Cuts at: 0 641 669
Size: 641 28
SacII CC_GC'GG
Cuts at: 0 342 669
Size: 342 327
SapI GCTCTTCn'nnn_
Cuts at: 0 322 669
Size: 322 347
SfcI C'TryA_G
Cuts at: 0 235 669
Size: 235 434
SfoI GGC'GCC
Cuts at: 0 273 669
Size: 273 396
SmlI C'TyrA_G
Cuts at: 0 634 669
Size: 634 35
StyI C'CwwG_G
Cuts at: 0 329 669
Size: 329 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 221 231 592 669
Size: 221 10 361 77
Fragments arranged by size:
361 221 77 10
TatI w'GTAC_w
Cuts at: 0 456 669
Size: 456 213
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 223 255 669
Size: 223 32 414
Fragments arranged by size:
414 223 32
Tth111I GACn'n_nGTC
Cuts at: 0 565 669
Size: 565 104
Enzymes that do cut:
AcuI AvaI BanI BanII BbeI BbsI BciVI BglII
Bme1580I BmrI BpuEI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BsrFI BssSI
BstXI BstYI BtgI BtsI CspCI EaeI EagI EarI
EcoICRI Eco57MI EcoO109I HaeII KasI MscI MslI MspA1I
NaeI NarI NgoMIV PasI PflMI PmlI PpuMI PshAI
PstI PvuII SacI SacII SapI SfcI SfoI SmlI
StyI TaqII TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BclI BfrBI BglI BlpI BmgBI BmtI BplI
BpmI Bpu10I BsaI BsaBI BsaWI BsiWI BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PfoI PmeI PpiI PsiI PspOMI PspXI PsrI PvuI
RsrII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI XbaI XcmI XhoI XmaI XmnI ZraI