(Linear) (Six-base) MAPSORT of: KIEE6196.seq  Check: 95  from: 1  to: 300



KIEE6196.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:28  ..

AccI GT'mk_AC

Cuts at:      0     250     300 
   Size:        250      50
 
AleI CACnn'nnGTG

Cuts at:      0     244     300 
   Size:        244      56
 
AlwNI CAG_nnn'CTG

Cuts at:      0     156     300 
   Size:        156     144
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      37     300 
   Size:         37     263
 
BmgBI CAC'GTC

Cuts at:      0     254     300 
   Size:        254      46
 
BseYI C'CCAG_C

Cuts at:      0      36     300 
   Size:         36     264
 
BsiHKAI G_wGCw'C

Cuts at:      0     180     300 
   Size:        180     120
 
BsmI GAATG_Cn'

Cuts at:      0      54     300 
   Size:         54     246
 
Bsp1286I G_dGCh'C

Cuts at:      0     180     300 
   Size:        180     120
 
BstYI r'GATC_y

Cuts at:      0     134     300 
   Size:        134     166
 
BtgI C'CryG_G

Cuts at:      0     101     300 
   Size:        101     199
 
EaeI y'GGCC_r

Cuts at:      0      80     236     300 
   Size:         80     156      64

  Fragments arranged by size: 

                 156      80      64
 
HincII GTy'rAC

Cuts at:      0     251     300 
   Size:        251      49
 
HindIII A'AGCT_T

Cuts at:      0     215     300 
   Size:        215      85
 
MscI TGG'CCA

Cuts at:      0     238     300 
   Size:        238      62
 
MslI CAynn'nnrTG

Cuts at:      0     244     300 
   Size:        244      56
 
SalI G'TCGA_C

Cuts at:      0     249     300 
   Size:        249      51
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     108     300 
   Size:        108     192
 

 Enzymes that do cut:

     AccI     AleI    AlwNI     BbsI    BmgBI    BseYI  BsiHKAI     BsmI
 Bsp1286I    BstYI     BtgI     EaeI   HincII  HindIII     MscI     MslI
     SalI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI