(Linear) (Six-base) MAPSORT of: KIEE6190.seq  Check: 5370  from: 1  to: 516



KIEE6190.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:28  ..

AgeI A'CCGG_T

Cuts at:      0     137     516 
   Size:        137     379
 
ApoI r'AATT_y

Cuts at:      0     128     516 
   Size:        128     388
 
AvaI C'yCGr_G

Cuts at:      0      14     516 
   Size:         14     502
 
BamHI G'GATC_C

Cuts at:      0     167     261     516 
   Size:        167      94     255

  Fragments arranged by size: 

                 255     167      94
 
BanI G'GyrC_C

Cuts at:      0     134     475     516 
   Size:        134     341      41

  Fragments arranged by size: 

                 341     134      41
 
BanII G_rGCy'C

Cuts at:      0      23     177     343     516 
   Size:         23     154     166     173

  Fragments arranged by size: 

                 173     166     154      23
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      81     516 
   Size:         81     435
 
BlpI GC'TnA_GC

Cuts at:      0     342     516 
   Size:        342     174
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     516 
   Size:        463      53
 
BsaWI w'CCGG_w

Cuts at:      0     137     516 
   Size:        137     379
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     385     516 
   Size:         19     366     131

  Fragments arranged by size: 

                 366     131      19
 
BseYI C'CCAG_C

Cuts at:      0     413     451     496     516 
   Size:        413      38      45      20

  Fragments arranged by size: 

                 413      45      38      20
 
Bsp1286I G_dGCh'C

Cuts at:      0      23     177     343     516 
   Size:         23     154     166     173

  Fragments arranged by size: 

                 173     166     154      23
 
BsrDI GCAATG_nn'

Cuts at:      0     120     353     516 
   Size:        120     233     163

  Fragments arranged by size: 

                 233     163     120
 
BsrFI r'CCGG_y

Cuts at:      0     137     516 
   Size:        137     379
 
BstYI r'GATC_y

Cuts at:      0     167     261     516 
   Size:        167      94     255

  Fragments arranged by size: 

                 255     167      94
 
Bsu36I CC'TnA_GG

Cuts at:      0     467     516 
   Size:        467      49
 
BtgI C'CryG_G

Cuts at:      0      28     332     516 
   Size:         28     304     184

  Fragments arranged by size: 

                 304     184      28
 
BtsI GCAGTG_nn'

Cuts at:      0     206     324     516 
   Size:        206     118     192

  Fragments arranged by size: 

                 206     192     118
 
EaeI y'GGCC_r

Cuts at:      0     329     516 
   Size:        329     187
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      98     516 
   Size:         98     418
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     516 
   Size:        463      53
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     376     516 
   Size:        376     140
 
EcoO109I rG'GnC_Cy

Cuts at:      0     210     447     516 
   Size:        210     237      69

  Fragments arranged by size: 

                 237     210      69
 
EcoRI G'AATT_C

Cuts at:      0     128     516 
   Size:        128     388
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     516 
   Size:        105     411
 
MscI TGG'CCA

Cuts at:      0     331     516 
   Size:        331     185
 
MspA1I CmG'CkG

Cuts at:      0     459     516 
   Size:        459      57
 
NcoI C'CATG_G

Cuts at:      0      28     332     516 
   Size:         28     304     184

  Fragments arranged by size: 

                 304     184      28
 
PfoI T'CCnGG_A

Cuts at:      0     440     516 
   Size:        440      76
 
PpuMI rG'GwC_Cy

Cuts at:      0     447     516 
   Size:        447      69
 
PvuII CAG'CTG

Cuts at:      0     459     516 
   Size:        459      57
 
StyI C'CwwG_G

Cuts at:      0      28     332     516 
   Size:         28     304     184

  Fragments arranged by size: 

                 304     184      28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      58     516 
   Size:         58     458
 

 Enzymes that do cut:

     AgeI     ApoI     AvaI    BamHI     BanI    BanII     BbsI     BlpI
     BpmI    BsaWI    BseRI    BseYI Bsp1286I    BsrDI    BsrFI    BstYI
   Bsu36I     BtgI     BtsI     EaeI     EciI  Eco57MI    EcoNI EcoO109I
    EcoRI     MmeI     MscI   MspA1I     NcoI     PfoI    PpuMI    PvuII
     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI  EcoICRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI