(Linear) (Six-base) MAPSORT of: KIEE6189.seq  Check: 5287  from: 1  to: 513



KIEE6189.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:28  ..

BamHI G'GATC_C

Cuts at:      0     297     513 
   Size:        297     216
 
BanII G_rGCy'C

Cuts at:      0      61     504     513 
   Size:         61     443       9

  Fragments arranged by size: 

                 443      61       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     357     513 
   Size:        357     156
 
BglI GCCn_nnn'nGGC

Cuts at:      0     310     513 
   Size:        310     203
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     507     513 
   Size:        507       6
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     513 
   Size:        106     407
 
Bsp1286I G_dGCh'C

Cuts at:      0      61     504     513 
   Size:         61     443       9

  Fragments arranged by size: 

                 443      61       9
 
BsrDI GCAATG_nn'

Cuts at:      0     335     513 
   Size:        335     178
 
BssSI C'ACGA_G

Cuts at:      0     200     513 
   Size:        200     313
 
BstYI r'GATC_y

Cuts at:      0     297     513 
   Size:        297     216
 
BtgI C'CryG_G

Cuts at:      0     308     513 
   Size:        308     205
 
BtsI GCAGTG_nn'

Cuts at:      0     148     513 
   Size:        148     365
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     232     264     513 
   Size:        232      32     249

  Fragments arranged by size: 

                 249     232      32
 
PfoI T'CCnGG_A

Cuts at:      0     191     513 
   Size:        191     322
 
SexAI A'CCwGG_T

Cuts at:      0     437     513 
   Size:        437      76
 
SfcI C'TryA_G

Cuts at:      0      74     513 
   Size:         74     439
 
SmlI C'TyrA_G

Cuts at:      0      85     513 
   Size:         85     428
 
StyI C'CwwG_G

Cuts at:      0     486     513 
   Size:        486      27
 
TatI w'GTAC_w

Cuts at:      0     476     513 
   Size:        476      37
 
Tth111I GACn'n_nGTC

Cuts at:      0     439     513 
   Size:        439      74
 

 Enzymes that do cut:

    BamHI    BanII     BbsI     BglI     BmrI    BpuEI Bsp1286I    BsrDI
    BssSI    BstYI     BtgI     BtsI    Hin4I     PfoI    SexAI     SfcI
     SmlI     StyI     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI