Restriction map for
pFN21AE6186
(Linear) (Six-base) MAPSORT of: KIEE6186.seq Check: 3032 from: 1 to: 504
KIEE6186.orf
With 182 enzymes: SgfI *
December 5, 2014 13:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 129 159 504
Size: 129 30 345
Fragments arranged by size:
345 129 30
AflIII A'CryG_T
Cuts at: 0 368 504
Size: 368 136
AleI CACnn'nnGTG
Cuts at: 0 56 139 504
Size: 56 83 365
Fragments arranged by size:
365 83 56
AlwNI CAG_nnn'CTG
Cuts at: 0 237 504
Size: 237 267
AvaI C'yCGr_G
Cuts at: 0 27 504
Size: 27 477
BanI G'GyrC_C
Cuts at: 0 154 274 504
Size: 154 120 230
Fragments arranged by size:
230 154 120
BglI GCCn_nnn'nGGC
Cuts at: 0 85 352 448 481 504
Size: 85 267 96 33 23
Fragments arranged by size:
267 96 85 33 23
Bme1580I G_kGCm'C
Cuts at: 0 182 224 504
Size: 182 42 280
Fragments arranged by size:
280 182 42
BmgBI CAC'GTC
Cuts at: 0 177 504
Size: 177 327
BsaAI yAC'GTr
Cuts at: 0 219 504
Size: 219 285
BsaBI GATnn'nnATC
Cuts at: 0 460 466 504
Size: 460 6 38
Fragments arranged by size:
460 38 6
BsaWI w'CCGG_w
Cuts at: 0 190 504
Size: 190 314
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 504
Size: 355 149
BsiEI CG_ry'CG
Cuts at: 0 244 504
Size: 244 260
Bsp1286I G_dGCh'C
Cuts at: 0 182 224 504
Size: 182 42 280
Fragments arranged by size:
280 182 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 129 159 222 504
Size: 129 30 63 282
Fragments arranged by size:
282 129 63 30
BstAPI GCAn_nnn'nTGC
Cuts at: 0 237 504
Size: 237 267
BstYI r'GATC_y
Cuts at: 0 455 461 504
Size: 455 6 43
Fragments arranged by size:
455 43 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 294 504
Size: 294 210
BtsI GCAGTG_nn'
Cuts at: 0 320 504
Size: 320 184
EaeI y'GGCC_r
Cuts at: 0 36 241 344 504
Size: 36 205 103 160
Fragments arranged by size:
205 160 103 36
EagI C'GGCC_G
Cuts at: 0 241 504
Size: 241 263
EarI CTCTTCn'nnn_
Cuts at: 0 333 344 504
Size: 333 11 160
Fragments arranged by size:
333 160 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 10 37 430 504
Size: 10 27 393 74
Fragments arranged by size:
393 74 27 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 490 504
Size: 490 14
EcoO109I rG'GnC_Cy
Cuts at: 0 234 483 495 504
Size: 234 249 12 9
Fragments arranged by size:
249 234 12 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 414 504
Size: 414 90
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 504
Size: 353 151
MslI CAynn'nnrTG
Cuts at: 0 56 139 313 504
Size: 56 83 174 191
Fragments arranged by size:
191 174 83 56
MspA1I CmG'CkG
Cuts at: 0 302 433 504
Size: 302 131 71
Fragments arranged by size:
302 131 71
NspI r_CATG'y
Cuts at: 0 265 372 504
Size: 265 107 132
Fragments arranged by size:
265 132 107
PciI A'CATG_T
Cuts at: 0 368 504
Size: 368 136
PmlI CAC'GTG
Cuts at: 0 219 504
Size: 219 285
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 288 310 320 342 504
Size: 288 22 10 22 162
Fragments arranged by size:
288 162 22 22 10
PpuMI rG'GwC_Cy
Cuts at: 0 234 504
Size: 234 270
PstI C_TGCA'G
Cuts at: 0 233 504
Size: 233 271
PvuII CAG'CTG
Cuts at: 0 433 504
Size: 433 71
SfcI C'TryA_G
Cuts at: 0 229 504
Size: 229 275
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 352 481 504
Size: 352 129 23
Fragments arranged by size:
352 129 23
SmaI CCC'GGG
Cuts at: 0 29 504
Size: 29 475
SphI G_CATG'C
Cuts at: 0 265 504
Size: 265 239
StyI C'CwwG_G
Cuts at: 0 389 504
Size: 389 115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 396 483 504
Size: 396 87 21
Fragments arranged by size:
396 87 21
XmaI C'CCGG_G
Cuts at: 0 27 504
Size: 27 477
Enzymes that do cut:
AarI AflIII AleI AlwNI AvaI BanI BglI Bme1580I
BmgBI BsaAI BsaBI BsaWI BsgI BsiEI Bsp1286I BspMI
BstAPI BstYI BtgZI BtsI EaeI EagI EarI EciI
EcoNI EcoO109I FalI MmeI MslI MspA1I NspI PciI
PmlI PpiI PpuMI PstI PvuII SfcI SfiI SmaI
SphI StyI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII DrdI
EcoICRI Eco57MI EcoRI EcoRV FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfoI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmnI ZraI