(Linear) (Six-base) MAPSORT of: KIEE6171.seq  Check: 509  from: 1  to: 249



KIEE6171.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:27  ..

ApoI r'AATT_y

Cuts at:      0     119     249 
   Size:        119     130
 
BsaWI w'CCGG_w

Cuts at:      0      84     249 
   Size:         84     165
 
BspEI T'CCGG_A

Cuts at:      0      84     249 
   Size:         84     165
 
BtsI GCAGTG_nn'

Cuts at:      0      50     249 
   Size:         50     199
 
DraI TTT'AAA

Cuts at:      0      72     249 
   Size:         72     177
 
EarI CTCTTCn'nnn_

Cuts at:      0      26     249 
   Size:         26     223
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      71     249 
   Size:         71     178
 
NdeI CA'TA_TG

Cuts at:      0     201     249 
   Size:        201      48
 
PfoI T'CCnGG_A

Cuts at:      0     104     249 
   Size:        104     145
 
SapI GCTCTTCn'nnn_

Cuts at:      0      26     249 
   Size:         26     223
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     213     249 
   Size:        213      36
 

 Enzymes that do cut:

     ApoI    BsaWI    BspEI     BtsI     DraI     EarI     FalI     NdeI
     PfoI     SapI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI