(Linear) (Six-base) MAPSORT of: KIEE6169.seq  Check: 4694  from: 1  to: 189



KIEE6169.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:27  ..

BsaHI Gr'CG_yC

Cuts at:      0      83     189 
   Size:         83     106
 
BsiEI CG_ry'CG

Cuts at:      0       8     189 
   Size:          8     181
 
BssHII G'CGCG_C

Cuts at:      0      99     189 
   Size:         99      90
 
BstBI TT'CG_AA

Cuts at:      0      22     189 
   Size:         22     167
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      78     189 
   Size:         78     111
 
EaeI y'GGCC_r

Cuts at:      0       5     189 
   Size:          5     184
 
EagI C'GGCC_G

Cuts at:      0       5     189 
   Size:          5     184
 
EcoO109I rG'GnC_Cy

Cuts at:      0      50     189 
   Size:         50     139
 
HincII GTy'rAC

Cuts at:      0      17     189 
   Size:         17     172
 
MslI CAynn'nnrTG

Cuts at:      0      76     176     189 
   Size:         76     100      13

  Fragments arranged by size: 

                 100      76      13
 
NotI GC'GGCC_GC

Cuts at:      0       5     189 
   Size:          5     184
 
PpuMI rG'GwC_Cy

Cuts at:      0      50     189 
   Size:         50     139
 
TatI w'GTAC_w

Cuts at:      0      29     189 
   Size:         29     160
 

 Enzymes that do cut:

    BsaHI    BsiEI   BssHII    BstBI    BstXI     EaeI     EagI EcoO109I
   HincII     MslI     NotI    PpuMI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI