(Linear) (Six-base) MAPSORT of: KIEE6164.seq  Check: 7900  from: 1  to: 267



KIEE6164.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     267 
   Size:        149     118
 
AlwNI CAG_nnn'CTG

Cuts at:      0      22     267 
   Size:         22     245
 
ApoI r'AATT_y

Cuts at:      0      46     267 
   Size:         46     221
 
BclI T'GATC_A

Cuts at:      0      24     267 
   Size:         24     243
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      70     127     267 
   Size:         70      57     140

  Fragments arranged by size: 

                 140      70      57
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      88     267 
   Size:         88     179
 
BsrDI GCAATG_nn'

Cuts at:      0     150     267 
   Size:        150     117
 
DraI TTT'AAA

Cuts at:      0     170     267 
   Size:        170      97
 
EaeI y'GGCC_r

Cuts at:      0     255     267 
   Size:        255      12
 
EarI CTCTTCn'nnn_

Cuts at:      0     125     267 
   Size:        125     142
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     267 
   Size:        149     118
 
EcoO109I rG'GnC_Cy

Cuts at:      0      56     267 
   Size:         56     211
 
MscI TGG'CCA

Cuts at:      0     257     267 
   Size:        257      10
 
PpuMI rG'GwC_Cy

Cuts at:      0      56     267 
   Size:         56     211
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     140     172     267 
   Size:        140      32      95

  Fragments arranged by size: 

                 140      95      32
 
SspI AAT'ATT

Cuts at:      0     166     267 
   Size:        166     101
 
StyI C'CwwG_G

Cuts at:      0     252     267 
   Size:        252      15
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BclI     BsaI    BspMI    BsrDI     DraI
     EaeI     EarI  Eco57MI EcoO109I     MscI    PpuMI     PsrI     SspI
     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI