(Linear) (Six-base) MAPSORT of: KIEE6157.seq  Check: 2845  from: 1  to: 216



KIEE6157.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:26  ..

BanII G_rGCy'C

Cuts at:      0      46     216 
   Size:         46     170
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     178     216 
   Size:        178      38
 
Bsp1286I G_dGCh'C

Cuts at:      0      46     216 
   Size:         46     170
 
EarI CTCTTCn'nnn_

Cuts at:      0      28      34     216 
   Size:         28       6     182

  Fragments arranged by size: 

                 182      28       6
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     216 
   Size:         80     136
 
SapI GCTCTTCn'nnn_

Cuts at:      0      34     216 
   Size:         34     182
 
SfcI C'TryA_G

Cuts at:      0     165     216 
   Size:        165      51
 
SspI AAT'ATT

Cuts at:      0     142     216 
   Size:        142      74
 
Tth111I GACn'n_nGTC

Cuts at:      0     100     216 
   Size:        100     116
 

 Enzymes that do cut:

    BanII     BbsI Bsp1286I     EarI     MmeI     SapI     SfcI     SspI
  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TatI     TstI     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI