(Linear) (Six-base) MAPSORT of: KIEE6156.seq  Check: 2616  from: 1  to: 396



KIEE6156.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     132     210     396 
   Size:         73      59      78     186

  Fragments arranged by size: 

                 186      78      73      59
 
AflIII A'CryG_T

Cuts at:      0     371     396 
   Size:        371      25
 
AlwNI CAG_nnn'CTG

Cuts at:      0     191     396 
   Size:        191     205
 
ApoI r'AATT_y

Cuts at:      0     274     396 
   Size:        274     122
 
AseI AT'TA_AT

Cuts at:      0      98     396 
   Size:         98     298
 
BglI GCCn_nnn'nGGC

Cuts at:      0     297     396 
   Size:        297      99
 
Bme1580I G_kGCm'C

Cuts at:      0     302     396 
   Size:        302      94
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     201     396 
   Size:        201     195
 
Bpu10I CC'TnA_GC

Cuts at:      0     340     349     396 
   Size:        340       9      47

  Fragments arranged by size: 

                 340      47       9
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     396 
   Size:         34     362
 
BseYI C'CCAG_C

Cuts at:      0     324     396 
   Size:        324      72
 
Bsp1286I G_dGCh'C

Cuts at:      0     302     396 
   Size:        302      94
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     137     396 
   Size:        137     259
 
BssSI C'ACGA_G

Cuts at:      0     301     396 
   Size:        301      95
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     324     396 
   Size:        324      72
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      54     396 
   Size:         54     342
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     132     210     396 
   Size:         73      59      78     186

  Fragments arranged by size: 

                 186      78      73      59
 
HincII GTy'rAC

Cuts at:      0      47     396 
   Size:         47     349
 
MslI CAynn'nnrTG

Cuts at:      0      31     396 
   Size:         31     365
 
NspI r_CATG'y

Cuts at:      0     375     396 
   Size:        375      21
 
PasI CC'CwG_GG

Cuts at:      0     232     396 
   Size:        232     164
 
PciI A'CATG_T

Cuts at:      0     371     396 
   Size:        371      25
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     232     264     396 
   Size:        232      32     132

  Fragments arranged by size: 

                 232     132      32
 
SmlI C'TyrA_G

Cuts at:      0      13     396 
   Size:         13     383
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI     AseI     BglI Bme1580I     BmrI
   Bpu10I    BpuEI    BseYI Bsp1286I    BspMI    BssSI    BstXI    BtgZI
  Eco57MI   HincII     MslI     NspI     PasI     PciI     PpiI     SmlI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI
     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI    PflMI     PfoI
     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI