(Linear) (Six-base) MAPSORT of: KIEE6155.seq  Check: 7916  from: 1  to: 390



KIEE6155.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     257     390 
   Size:        257     133
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     390 
   Size:         55     335
 
AflIII A'CryG_T

Cuts at:      0      49     390 
   Size:         49     341
 
ApaLI G'TGCA_C

Cuts at:      0      91     390 
   Size:         91     299
 
AvaI C'yCGr_G

Cuts at:      0      32     390 
   Size:         32     358
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      61     232     364     390 
   Size:         61     171     132      26

  Fragments arranged by size: 

                 171     132      61      26
 
BglII A'GATC_T

Cuts at:      0     185     390 
   Size:        185     205
 
Bme1580I G_kGCm'C

Cuts at:      0      95     390 
   Size:         95     295
 
BmgBI CAC'GTC

Cuts at:      0      10     390 
   Size:         10     380
 
BmtI G_CTAG'C

Cuts at:      0     199     259     390 
   Size:        199      60     131

  Fragments arranged by size: 

                 199     131      60
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     390 
   Size:        128     262
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      91     289     390 
   Size:         91     198     101

  Fragments arranged by size: 

                 198     101      91
 
BsiEI CG_ry'CG

Cuts at:      0     182     390 
   Size:        182     208
 
BsiHKAI G_wGCw'C

Cuts at:      0      95     390 
   Size:         95     295
 
Bsp1286I G_dGCh'C

Cuts at:      0      95     390 
   Size:         95     295
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     257     390 
   Size:        257     133
 
BsrFI r'CCGG_y

Cuts at:      0      64     136     390 
   Size:         64      72     254

  Fragments arranged by size: 

                 254      72      64
 
BstYI r'GATC_y

Cuts at:      0     185     390 
   Size:        185     205
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     291     390 
   Size:        291      99
 
EaeI y'GGCC_r

Cuts at:      0      84     179     390 
   Size:         84      95     211

  Fragments arranged by size: 

                 211      95      84
 
EagI C'GGCC_G

Cuts at:      0     179     390 
   Size:        179     211
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     128     390 
   Size:         55      73     262

  Fragments arranged by size: 

                 262      73      55
 
HaeII r_GCGC'y

Cuts at:      0     143     390 
   Size:        143     247
 
HindIII A'AGCT_T

Cuts at:      0     286     390 
   Size:        286     104
 
MluI A'CGCG_T

Cuts at:      0      49     390 
   Size:         49     341
 
MspA1I CmG'CkG

Cuts at:      0     160     390 
   Size:        160     230
 
NaeI GCC'GGC

Cuts at:      0     138     390 
   Size:        138     252
 
NgoMIV G'CCGG_C

Cuts at:      0     136     390 
   Size:        136     254
 
NheI G'CTAG_C

Cuts at:      0     195     255     390 
   Size:        195      60     135

  Fragments arranged by size: 

                 195     135      60
 
NruI TCG'CGA

Cuts at:      0     215     390 
   Size:        215     175
 
PstI C_TGCA'G

Cuts at:      0     254     390 
   Size:        254     136
 
SfcI C'TryA_G

Cuts at:      0     250     390 
   Size:        250     140
 
StyI C'CwwG_G

Cuts at:      0     380     390 
   Size:        380      10
 

 Enzymes that do cut:

     AarI     AcuI   AflIII    ApaLI     AvaI     BbsI    BglII Bme1580I
    BmgBI     BmtI     BpmI    BseRI    BsiEI  BsiHKAI Bsp1286I    BspMI
    BsrFI    BstYI    BtgZI     EaeI     EagI  Eco57MI    HaeII  HindIII
     MluI   MspA1I     NaeI   NgoMIV     NheI     NruI     PstI     SfcI
     StyI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI     BmrI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII     HpaI     KasI
     KpnI     MfeI     MmeI     MscI     MslI     NarI     NcoI     NdeI
     NotI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI