(Linear) (Six-base) MAPSORT of: KIEE6154.seq  Check: 5071  from: 1  to: 234



KIEE6154.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     234 
   Size:         83     151
 
AleI CACnn'nnGTG

Cuts at:      0      70     234 
   Size:         70     164
 
AseI AT'TA_AT

Cuts at:      0     149     234 
   Size:        149      85
 
BclI T'GATC_A

Cuts at:      0     206     234 
   Size:        206      28
 
BsrDI GCAATG_nn'

Cuts at:      0      83     234 
   Size:         83     151
 
BssSI C'ACGA_G

Cuts at:      0     167     234 
   Size:        167      67
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      72     234 
   Size:         72     162
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     175     234 
   Size:        175      59
 
EarI CTCTTCn'nnn_

Cuts at:      0      99     234 
   Size:         99     135
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     234 
   Size:         83     151
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     180     234 
   Size:        180      54
 
MslI CAynn'nnrTG

Cuts at:      0      70     234 
   Size:         70     164
 

 Enzymes that do cut:

     AcuI     AleI     AseI     BclI    BsrDI    BssSI    BstXI    BtgZI
     EarI  Eco57MI     MmeI     MslI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI