(Linear) (Six-base) MAPSORT of: KIEE6147.seq  Check: 1760  from: 1  to: 435



KIEE6147.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:26  ..

AccI GT'mk_AC

Cuts at:      0     395     435 
   Size:        395      40
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     435 
   Size:         42     393
 
ApoI r'AATT_y

Cuts at:      0      85     435 
   Size:         85     350
 
BclI T'GATC_A

Cuts at:      0     264     435 
   Size:        264     171
 
Bme1580I G_kGCm'C

Cuts at:      0     152     435 
   Size:        152     283
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     435 
   Size:        337      98
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     256     435 
   Size:        256     179
 
BsiHKAI G_wGCw'C

Cuts at:      0       7     316     435 
   Size:          7     309     119

  Fragments arranged by size: 

                 309     119       7
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     152     316     435 
   Size:          7     145     164     119

  Fragments arranged by size: 

                 164     145     119       7
 
BsrGI T'GTAC_A

Cuts at:      0      77     435 
   Size:         77     358
 
BstZ17I GTA'TAC

Cuts at:      0     396     435 
   Size:        396      39
 
BtgI C'CryG_G

Cuts at:      0     144     435 
   Size:        144     291
 
EaeI y'GGCC_r

Cuts at:      0      18     141     435 
   Size:         18     123     294

  Fragments arranged by size: 

                 294     123      18
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     337     435 
   Size:         42     295      98

  Fragments arranged by size: 

                 295      98      42
 
MscI TGG'CCA

Cuts at:      0     143     435 
   Size:        143     292
 
NcoI C'CATG_G

Cuts at:      0     144     435 
   Size:        144     291
 
PsiI TTA'TAA

Cuts at:      0     158     383     425     435 
   Size:        158     225      42      10

  Fragments arranged by size: 

                 225     158      42      10
 
SfcI C'TryA_G

Cuts at:      0     368     435 
   Size:        368      67
 
SspI AAT'ATT

Cuts at:      0     414     435 
   Size:        414      21
 
StyI C'CwwG_G

Cuts at:      0     144     435 
   Size:        144     291
 
TatI w'GTAC_w

Cuts at:      0      77     174     435 
   Size:         77      97     261

  Fragments arranged by size: 

                 261      97      77
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     BclI Bme1580I     BpmI    BseRI  BsiHKAI
 Bsp1286I    BsrGI  BstZ17I     BtgI     EaeI  Eco57MI     MscI     NcoI
     PsiI     SfcI     SspI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI