(Linear) (Six-base) MAPSORT of: KIEE6146.seq  Check: 8155  from: 1  to: 294



KIEE6146.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:26  ..

BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     147     180     294 
   Size:        147      33     114

  Fragments arranged by size: 

                 147     114      33
 
BamHI G'GATC_C

Cuts at:      0     126     294 
   Size:        126     168
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     294 
   Size:        283      11
 
Bpu10I CC'TnA_GC

Cuts at:      0     121     294 
   Size:        121     173
 
BsrDI GCAATG_nn'

Cuts at:      0     147     294 
   Size:        147     147
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      13     294 
   Size:         13     281
 
BstYI r'GATC_y

Cuts at:      0     126     294 
   Size:        126     168
 
BtgI C'CryG_G

Cuts at:      0     130     294 
   Size:        130     164
 
DraI TTT'AAA

Cuts at:      0     204     294 
   Size:        204      90
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     294 
   Size:        283      11
 
HindIII A'AGCT_T

Cuts at:      0     146     294 
   Size:        146     148
 
MslI CAynn'nnrTG

Cuts at:      0      11      17      23     294 
   Size:         11       6       6     271

  Fragments arranged by size: 

                 271      11       6       6
 
NcoI C'CATG_G

Cuts at:      0     130     294 
   Size:        130     164
 
SfcI C'TryA_G

Cuts at:      0     242     294 
   Size:        242      52
 
SspI AAT'ATT

Cuts at:      0     256     294 
   Size:        256      38
 
StyI C'CwwG_G

Cuts at:      0     130     294 
   Size:        130     164
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     151     294 
   Size:        151     143
 

 Enzymes that do cut:

     BaeI    BamHI     BpmI   Bpu10I    BsrDI    BstXI    BstYI     BtgI
     DraI  Eco57MI  HindIII     MslI     NcoI     SfcI     SspI     StyI
    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI