(Linear) (Six-base) MAPSORT of: KIEE6143.seq  Check: 7735  from: 1  to: 183



KIEE6143.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      49     183 
   Size:         49     134
 
AlwNI CAG_nnn'CTG

Cuts at:      0     176     183 
   Size:        176       7
 
Bme1580I G_kGCm'C

Cuts at:      0     135     183 
   Size:        135      48
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      21      27     183 
   Size:         21       6     156

  Fragments arranged by size: 

                 156      21       6
 
Bsp1286I G_dGCh'C

Cuts at:      0     135     183 
   Size:        135      48
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      49     183 
   Size:         49     134
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      68      83     183 
   Size:         68      15     100

  Fragments arranged by size: 

                 100      68      15
 
EarI CTCTTCn'nnn_

Cuts at:      0      85     183 
   Size:         85      98
 
MspA1I CmG'CkG

Cuts at:      0     173     183 
   Size:        173      10
 
PasI CC'CwG_GG

Cuts at:      0     135     183 
   Size:        135      48
 
PvuII CAG'CTG

Cuts at:      0     173     183 
   Size:        173      10
 
SapI GCTCTTCn'nnn_

Cuts at:      0      85     183 
   Size:         85      98
 

 Enzymes that do cut:

     AarI    AlwNI Bme1580I     BsgI Bsp1286I    BspMI   BstAPI     EarI
   MspA1I     PasI    PvuII     SapI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI