(Linear) (Six-base) MAPSORT of: KIEE6141.seq  Check: 8274  from: 1  to: 303



KIEE6141.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:25  ..

BanI G'GyrC_C

Cuts at:      0     256     295     303 
   Size:        256      39       8

  Fragments arranged by size: 

                 256      39       8
 
BglII A'GATC_T

Cuts at:      0     236     303 
   Size:        236      67
 
BlpI GC'TnA_GC

Cuts at:      0     177     303 
   Size:        177     126
 
Bme1580I G_kGCm'C

Cuts at:      0     261     298     303 
   Size:        261      37       5

  Fragments arranged by size: 

                 261      37       5
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     303 
   Size:         28     275
 
BsaBI GATnn'nnATC

Cuts at:      0     226     303 
   Size:        226      77
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       1      31     259     289     303 
   Size:          1      30     228      30      14

  Fragments arranged by size: 

                 228      30      30      14       1
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      40     303 
   Size:         40     263
 
BseYI C'CCAG_C

Cuts at:      0     132     251     303 
   Size:        132     119      52

  Fragments arranged by size: 

                 132     119      52
 
BsiHKAI G_wGCw'C

Cuts at:      0     212     303 
   Size:        212      91
 
Bsp1286I G_dGCh'C

Cuts at:      0     212     261     298     303 
   Size:        212      49      37       5

  Fragments arranged by size: 

                 212      49      37       5
 
BsrFI r'CCGG_y

Cuts at:      0     242     303 
   Size:        242      61
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     291     303 
   Size:        291      12
 
BstYI r'GATC_y

Cuts at:      0     236     303 
   Size:        236      67
 
EarI CTCTTCn'nnn_

Cuts at:      0     199     303 
   Size:        199     104
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     303 
   Size:         28     275
 
HaeII r_GCGC'y

Cuts at:      0     177     303 
   Size:        177     126
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       1      33     259     291     303 
   Size:          1      32     226      32      12

  Fragments arranged by size: 

                 226      32      32      12       1
 
HincII GTy'rAC

Cuts at:      0      96     303 
   Size:         96     207
 
MspA1I CmG'CkG

Cuts at:      0     181     251     303 
   Size:        181      70      52

  Fragments arranged by size: 

                 181      70      52
 
PasI CC'CwG_GG

Cuts at:      0     291     303 
   Size:        291      12
 
PfoI T'CCnGG_A

Cuts at:      0     217     303 
   Size:        217      86
 
PvuII CAG'CTG

Cuts at:      0     181     303 
   Size:        181     122
 
ScaI AGT'ACT

Cuts at:      0      52     303 
   Size:         52     251
 
StuI AGG'CCT

Cuts at:      0     121     303 
   Size:        121     182
 
StyI C'CwwG_G

Cuts at:      0     261     303 
   Size:        261      42
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     189     303 
   Size:        189     114
 
TatI w'GTAC_w

Cuts at:      0      50     303 
   Size:         50     253
 

 Enzymes that do cut:

     BanI    BglII     BlpI Bme1580I     BpmI    BsaBI    BsaXI    BseRI
    BseYI  BsiHKAI Bsp1286I    BsrFI    BstXI    BstYI     EarI  Eco57MI
    HaeII    Hin4I   HincII   MspA1I     PasI     PfoI    PvuII     ScaI
     StuI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI