Restriction map for
pFN21AE6140
(Linear) (Six-base) MAPSORT of: KIEE6140.seq Check: 2411 from: 1 to: 510
KIEE6140.orf
With 182 enzymes: SgfI *
December 5, 2014 13:25 ..
AatII G_ACGT'C
Cuts at: 0 406 510
Size: 406 104
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 510
Size: 299 211
AflIII A'CryG_T
Cuts at: 0 474 510
Size: 474 36
AhdI GACnn_n'nnGTC
Cuts at: 0 402 510
Size: 402 108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 181 510
Size: 181 329
AvrII C'CTAG_G
Cuts at: 0 231 510
Size: 231 279
BanI G'GyrC_C
Cuts at: 0 346 510
Size: 346 164
BanII G_rGCy'C
Cuts at: 0 185 510
Size: 185 325
BbsI GAAGACnn'nnnn_
Cuts at: 0 287 510
Size: 287 223
BglI GCCn_nnn'nGGC
Cuts at: 0 103 510
Size: 103 407
Bme1580I G_kGCm'C
Cuts at: 0 99 453 510
Size: 99 354 57
Fragments arranged by size:
354 99 57
BmgBI CAC'GTC
Cuts at: 0 261 393 477 510
Size: 261 132 84 33
Fragments arranged by size:
261 132 84 33
Bpu10I CC'TnA_GC
Cuts at: 0 133 510
Size: 133 377
BsaBI GATnn'nnATC
Cuts at: 0 505 510
Size: 505 5
BsaHI Gr'CG_yC
Cuts at: 0 239 403 456 510
Size: 239 164 53 54
Fragments arranged by size:
239 164 54 53
BsaWI w'CCGG_w
Cuts at: 0 491 510
Size: 491 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 54 57 69 72 510
Size: 54 3 12 3 438
Fragments arranged by size:
438 54 12 3 3
BseYI C'CCAG_C
Cuts at: 0 195 510
Size: 195 315
BsmBI CGTCTCn'nnnn_
Cuts at: 0 230 510
Size: 230 280
Bsp1286I G_dGCh'C
Cuts at: 0 99 185 453 510
Size: 99 86 268 57
Fragments arranged by size:
268 99 86 57
BspEI T'CCGG_A
Cuts at: 0 491 510
Size: 491 19
BspHI T'CATG_A
Cuts at: 0 353 510
Size: 353 157
BsrBI CCG'CTC
Cuts at: 0 31 510
Size: 31 479
BstEII G'GTnAC_C
Cuts at: 0 408 510
Size: 408 102
BtgI C'CryG_G
Cuts at: 0 119 510
Size: 119 391
DrdI GACnn_nn'nnGTC
Cuts at: 0 289 349 510
Size: 289 60 161
Fragments arranged by size:
289 161 60
EarI CTCTTCn'nnn_
Cuts at: 0 21 57 75 510
Size: 21 36 18 435
Fragments arranged by size:
435 36 21 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 510
Size: 299 211
EcoO109I rG'GnC_Cy
Cuts at: 0 111 129 510
Size: 111 18 381
Fragments arranged by size:
381 111 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 388 402 420 434 510
Size: 388 14 18 14 76
Fragments arranged by size:
388 76 18 14 14
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 510
Size: 326 184
MslI CAynn'nnrTG
Cuts at: 0 316 352 510
Size: 316 36 158
Fragments arranged by size:
316 158 36
MspA1I CmG'CkG
Cuts at: 0 381 510
Size: 381 129
NcoI C'CATG_G
Cuts at: 0 119 510
Size: 119 391
NspI r_CATG'y
Cuts at: 0 141 510
Size: 141 369
PpuMI rG'GwC_Cy
Cuts at: 0 111 510
Size: 111 399
PvuII CAG'CTG
Cuts at: 0 381 510
Size: 381 129
StyI C'CwwG_G
Cuts at: 0 119 231 510
Size: 119 112 279
Fragments arranged by size:
279 119 112
XmnI GAAnn'nnTTC
Cuts at: 0 15 361 510
Size: 15 346 149
Fragments arranged by size:
346 149 15
ZraI GAC'GTC
Cuts at: 0 404 510
Size: 404 106
Enzymes that do cut:
AatII AcuI AflIII AhdI AlfI AvrII BanI BanII
BbsI BglI Bme1580I BmgBI Bpu10I BsaBI BsaHI BsaWI
BseRI BseYI BsmBI Bsp1286I BspEI BspHI BsrBI BstEII
BtgI DrdI EarI Eco57MI EcoO109I Hin4I MmeI MslI
MspA1I NcoI NspI PpuMI PvuII StyI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI
BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII EaeI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI