(Linear) (Six-base) MAPSORT of: KIEE6132.seq  Check: 9858  from: 1  to: 342



KIEE6132.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:25  ..

AleI CACnn'nnGTG

Cuts at:      0      40     342 
   Size:         40     302
 
ApoI r'AATT_y

Cuts at:      0     197     342 
   Size:        197     145
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     267     301     342 
   Size:        267      34      41

  Fragments arranged by size: 

                 267      41      34
 
BfrBI ATG'CAT

Cuts at:      0      58     113     342 
   Size:         58      55     229

  Fragments arranged by size: 

                 229      58      55
 
BsrDI GCAATG_nn'

Cuts at:      0      60     342 
   Size:         60     282
 
BstYI r'GATC_y

Cuts at:      0     117     342 
   Size:        117     225
 
EcoO109I rG'GnC_Cy

Cuts at:      0     303     342 
   Size:        303      39
 
MslI CAynn'nnrTG

Cuts at:      0      40     342 
   Size:         40     302
 
NsiI A_TGCA'T

Cuts at:      0      60     115     342 
   Size:         60      55     227

  Fragments arranged by size: 

                 227      60      55
 
NspI r_CATG'y

Cuts at:      0     179     342 
   Size:        179     163
 
PfoI T'CCnGG_A

Cuts at:      0      90     342 
   Size:         90     252
 
PpuMI rG'GwC_Cy

Cuts at:      0     303     342 
   Size:        303      39
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     152     342 
   Size:        152     190
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     111     342 
   Size:        111     231
 

 Enzymes that do cut:

     AleI     ApoI     BcgI    BfrBI    BsrDI    BstYI EcoO109I     MslI
     NsiI     NspI     PfoI    PpuMI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI