(Linear) (Six-base) MAPSORT of: KIEE6131.seq  Check: 1382  from: 1  to: 189



KIEE6131.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:25  ..

ApaI G_GGCC'C

Cuts at:      0      67     189 
   Size:         67     122
 
ApoI r'AATT_y

Cuts at:      0      75     189 
   Size:         75     114
 
BanII G_rGCy'C

Cuts at:      0      67     189 
   Size:         67     122
 
Bme1580I G_kGCm'C

Cuts at:      0      67     189 
   Size:         67     122
 
BsaWI w'CCGG_w

Cuts at:      0      17     189 
   Size:         17     172
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      73     189 
   Size:         73     116
 
Bsp1286I G_dGCh'C

Cuts at:      0      67     189 
   Size:         67     122
 
BspEI T'CCGG_A

Cuts at:      0      17     189 
   Size:         17     172
 
BstYI r'GATC_y

Cuts at:      0     136     189 
   Size:        136      53
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      17      52     189 
   Size:         17      35     137

  Fragments arranged by size: 

                 137      35      17
 
EcoO109I rG'GnC_Cy

Cuts at:      0      63      64     189 
   Size:         63       1     125

  Fragments arranged by size: 

                 125      63       1
 
PasI CC'CwG_GG

Cuts at:      0      67     189 
   Size:         67     122
 
PspOMI G'GGCC_C

Cuts at:      0      63     189 
   Size:         63     126
 
TatI w'GTAC_w

Cuts at:      0     128     189 
   Size:        128      61
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      85     117     189 
   Size:         85      32      72

  Fragments arranged by size: 

                  85      72      32
 

 Enzymes that do cut:

     ApaI     ApoI    BanII Bme1580I    BsaWI    BseRI Bsp1286I    BspEI
    BstYI    CspCI EcoO109I     PasI   PspOMI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI