(Linear) (Six-base) MAPSORT of: KIEE6130.seq  Check: 7923  from: 1  to: 423



KIEE6130.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:25  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     423 
   Size:        343      80
 
AlwNI CAG_nnn'CTG

Cuts at:      0      60     423 
   Size:         60     363
 
BanII G_rGCy'C

Cuts at:      0     383     423 
   Size:        383      40
 
BbvCI CC'TCA_GC

Cuts at:      0     121     423 
   Size:        121     302
 
BlpI GC'TnA_GC

Cuts at:      0      61     423 
   Size:         61     362
 
Bpu10I CC'TnA_GC

Cuts at:      0     121     423 
   Size:        121     302
 
BsaWI w'CCGG_w

Cuts at:      0      96     423 
   Size:         96     327
 
BseYI C'CCAG_C

Cuts at:      0     229     265     423 
   Size:        229      36     158

  Fragments arranged by size: 

                 229     158      36
 
BsiEI CG_ry'CG

Cuts at:      0      97     423 
   Size:         97     326
 
BsiHKAI G_wGCw'C

Cuts at:      0      49     423 
   Size:         49     374
 
Bsp1286I G_dGCh'C

Cuts at:      0      49     383     423 
   Size:         49     334      40

  Fragments arranged by size: 

                 334      49      40
 
BtgI C'CryG_G

Cuts at:      0     374     423 
   Size:        374      49
 
BtsI GCAGTG_nn'

Cuts at:      0      86     423 
   Size:         86     337
 
EarI CTCTTCn'nnn_

Cuts at:      0     340     423 
   Size:        340      83
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     423 
   Size:        343      80
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     367     423 
   Size:        367      56
 
HaeII r_GCGC'y

Cuts at:      0     188     423 
   Size:        188     235
 
MspA1I CmG'CkG

Cuts at:      0      57     423 
   Size:         57     366
 
NcoI C'CATG_G

Cuts at:      0     374     423 
   Size:        374      49
 
PstI C_TGCA'G

Cuts at:      0      81     423 
   Size:         81     342
 
PvuII CAG'CTG

Cuts at:      0      57     423 
   Size:         57     366
 
SapI GCTCTTCn'nnn_

Cuts at:      0     340     423 
   Size:        340      83
 
SfcI C'TryA_G

Cuts at:      0      77     423 
   Size:         77     346
 
StyI C'CwwG_G

Cuts at:      0      35     374     423 
   Size:         35     339      49

  Fragments arranged by size: 

                 339      49      35
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      33     246     423 
   Size:         33     213     177

  Fragments arranged by size: 

                 213     177      33
 

 Enzymes that do cut:

     AcuI    AlwNI    BanII    BbvCI     BlpI   Bpu10I    BsaWI    BseYI
    BsiEI  BsiHKAI Bsp1286I     BtgI     BtsI     EarI  Eco57MI    EcoNI
    HaeII   MspA1I     NcoI     PstI    PvuII     SapI     SfcI     StyI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI     BsgI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI