(Linear) (Six-base) MAPSORT of: KIEE6129.seq  Check: 2531  from: 1  to: 450



KIEE6129.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:25  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     450 
   Size:         83     367
 
AleI CACnn'nnGTG

Cuts at:      0      70     400     450 
   Size:         70     330      50

  Fragments arranged by size: 

                 330      70      50
 
ApoI r'AATT_y

Cuts at:      0     191     450 
   Size:        191     259
 
BanII G_rGCy'C

Cuts at:      0     177     450 
   Size:        177     273
 
BclI T'GATC_A

Cuts at:      0     444     450 
   Size:        444       6
 
BmgBI CAC'GTC

Cuts at:      0     402     450 
   Size:        402      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     450 
   Size:        200     250
 
BsaWI w'CCGG_w

Cuts at:      0     229     450 
   Size:        229     221
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     224     254     450 
   Size:        224      30     196

  Fragments arranged by size: 

                 224     196      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     177     450 
   Size:        177     273
 
Bsp1286I G_dGCh'C

Cuts at:      0     177     450 
   Size:        177     273
 
BspHI T'CATG_A

Cuts at:      0     177     450 
   Size:        177     273
 
BsrDI GCAATG_nn'

Cuts at:      0      83     450 
   Size:         83     367
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      72     450 
   Size:         72     378
 
EarI CTCTTCn'nnn_

Cuts at:      0      99     215     450 
   Size:         99     116     235

  Fragments arranged by size: 

                 235     116      99
 
EcoICRI GAG'CTC

Cuts at:      0     175     450 
   Size:        175     275
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     200     450 
   Size:         83     117     250

  Fragments arranged by size: 

                 250     117      83
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     429     450 
   Size:        429      21
 
MfeI C'AATT_G

Cuts at:      0      38     450 
   Size:         38     412
 
MslI CAynn'nnrTG

Cuts at:      0      70     400     450 
   Size:         70     330      50

  Fragments arranged by size: 

                 330      70      50
 
NdeI CA'TA_TG

Cuts at:      0     156     450 
   Size:        156     294
 
SacI G_AGCT'C

Cuts at:      0     177     450 
   Size:        177     273
 

 Enzymes that do cut:

     AcuI     AleI     ApoI    BanII     BclI    BmgBI     BpmI    BsaWI
    BsaXI  BsiHKAI Bsp1286I    BspHI    BsrDI    BstXI     EarI  EcoICRI
  Eco57MI    Hin4I     MfeI     MslI     NdeI     SacI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI