(Linear) (Six-base) MAPSORT of: KIEE6126.seq  Check: 2404  from: 1  to: 315



KIEE6126.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     115     315 
   Size:        115     200
 
Acc65I G'GTAC_C

Cuts at:      0     278     315 
   Size:        278      37
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     315 
   Size:        192     123
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     123     315 
   Size:        123     192
 
ApaLI G'TGCA_C

Cuts at:      0     147     315 
   Size:        147     168
 
BanI G'GyrC_C

Cuts at:      0       7     103     278     315 
   Size:          7      96     175      37

  Fragments arranged by size: 

                 175      96      37       7
 
BclI T'GATC_A

Cuts at:      0      62     315 
   Size:         62     253
 
BglI GCCn_nnn'nGGC

Cuts at:      0     185     315 
   Size:        185     130
 
Bme1580I G_kGCm'C

Cuts at:      0     151     315 
   Size:        151     164
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     243     315 
   Size:        243      72
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     271     301     315 
   Size:        271      30      14

  Fragments arranged by size: 

                 271      30      14
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22     187     315 
   Size:         22     165     128

  Fragments arranged by size: 

                 165     128      22
 
BseYI C'CCAG_C

Cuts at:      0     156     315 
   Size:        156     159
 
BsiHKAI G_wGCw'C

Cuts at:      0     151     259     315 
   Size:        151     108      56

  Fragments arranged by size: 

                 151     108      56
 
Bsp1286I G_dGCh'C

Cuts at:      0     151     259     315 
   Size:        151     108      56

  Fragments arranged by size: 

                 151     108      56
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     115     257     266     315 
   Size:        115     142       9      49

  Fragments arranged by size: 

                 142     115      49       9
 
BsrBI CCG'CTC

Cuts at:      0      81     315 
   Size:         81     234
 
BstYI r'GATC_y

Cuts at:      0     197     315 
   Size:        197     118
 
BtgI C'CryG_G

Cuts at:      0     290     315 
   Size:        290      25
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     243     315 
   Size:        192      51      72

  Fragments arranged by size: 

                 192      72      51
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     207     315 
   Size:        207     108
 
EcoO109I rG'GnC_Cy

Cuts at:      0      71     315 
   Size:         71     244
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     219     315 
   Size:        219      96
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       8      40     315 
   Size:          8      32     275

  Fragments arranged by size: 

                 275      32       8
 
KpnI G_GTAC'C

Cuts at:      0     282     315 
   Size:        282      33
 
MspA1I CmG'CkG

Cuts at:      0     156     284     315 
   Size:        156     128      31

  Fragments arranged by size: 

                 156     128      31
 
NcoI C'CATG_G

Cuts at:      0     290     315 
   Size:        290      25
 
PfoI T'CCnGG_A

Cuts at:      0     266     315 
   Size:        266      49
 
StyI C'CwwG_G

Cuts at:      0     290     315 
   Size:        290      25
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      76     108     315 
   Size:         76      32     207

  Fragments arranged by size: 

                 207      76      32
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AlfI    ApaLI     BanI     BclI     BglI
 Bme1580I     BpmI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BspMI
    BsrBI    BstYI     BtgI  Eco57MI    EcoNI EcoO109I     FalI    Hin4I
     KpnI   MspA1I     NcoI     PfoI     StyI     TstI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI