(Linear) (Six-base) MAPSORT of: KIEE6123.seq  Check: 1864  from: 1  to: 453



KIEE6123.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     453 
   Size:        373      80
 
AgeI A'CCGG_T

Cuts at:      0      60     453 
   Size:         60     393
 
BanII G_rGCy'C

Cuts at:      0     121     453 
   Size:        121     332
 
BlpI GC'TnA_GC

Cuts at:      0      37     145     453 
   Size:         37     108     308

  Fragments arranged by size: 

                 308     108      37
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     238     424     453 
   Size:        238     186      29

  Fragments arranged by size: 

                 238     186      29
 
Bpu10I CC'TnA_GC

Cuts at:      0     263     453 
   Size:        263     190
 
BsaHI Gr'CG_yC

Cuts at:      0     239     453 
   Size:        239     214
 
BsaWI w'CCGG_w

Cuts at:      0      60     453 
   Size:         60     393
 
BseYI C'CCAG_C

Cuts at:      0     419     453 
   Size:        419      34
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     453 
   Size:         85     368
 
Bsp1286I G_dGCh'C

Cuts at:      0     121     453 
   Size:        121     332
 
BsrFI r'CCGG_y

Cuts at:      0      60     453 
   Size:         60     393
 
BtgI C'CryG_G

Cuts at:      0     215     453 
   Size:        215     238
 
EarI CTCTTCn'nnn_

Cuts at:      0     418     453 
   Size:        418      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     453 
   Size:        373      80
 
HaeII r_GCGC'y

Cuts at:      0      37     453 
   Size:         37     416
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     429     453 
   Size:        429      24
 
MslI CAynn'nnrTG

Cuts at:      0     232     453 
   Size:        232     221
 
MspA1I CmG'CkG

Cuts at:      0      46     144     453 
   Size:         46      98     309

  Fragments arranged by size: 

                 309      98      46
 
PsiI TTA'TAA

Cuts at:      0     100     453 
   Size:        100     353
 
PvuII CAG'CTG

Cuts at:      0     144     453 
   Size:        144     309
 
SfcI C'TryA_G

Cuts at:      0     318     453 
   Size:        318     135
 
SspI AAT'ATT

Cuts at:      0     192     453 
   Size:        192     261
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     145     453 
   Size:        145     308
 
TatI w'GTAC_w

Cuts at:      0     432     453 
   Size:        432      21
 

 Enzymes that do cut:

     AcuI     AgeI    BanII     BlpI     BmrI   Bpu10I    BsaHI    BsaWI
    BseYI     BsgI Bsp1286I    BsrFI     BtgI     EarI  Eco57MI    HaeII
    Hin4I     MslI   MspA1I     PsiI    PvuII     SfcI     SspI    TaqII
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII Bme1580I    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI