(Linear) (Six-base) MAPSORT of: KIEE6122.seq  Check: 9898  from: 1  to: 258



KIEE6122.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:24  ..

Acc65I G'GTAC_C

Cuts at:      0     167     258 
   Size:        167      91
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     258 
   Size:        254       4
 
AvaI C'yCGr_G

Cuts at:      0     247     258 
   Size:        247      11
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     159     192     258 
   Size:        159      33      66

  Fragments arranged by size: 

                 159      66      33
 
BanI G'GyrC_C

Cuts at:      0     167     258 
   Size:        167      91
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      14     258 
   Size:         14     244
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     225     258 
   Size:        225      33
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      10     258 
   Size:         10     248
 
BsrDI GCAATG_nn'

Cuts at:      0     134     258 
   Size:        134     124
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     258 
   Size:         19     239
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     258 
   Size:        254       4
 
EcoRV GAT'ATC

Cuts at:      0     150     258 
   Size:        150     108
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     134     166     258 
   Size:        134      32      92

  Fragments arranged by size: 

                 134      92      32
 
KpnI G_GTAC'C

Cuts at:      0     171     258 
   Size:        171      87
 
MspA1I CmG'CkG

Cuts at:      0     116     258 
   Size:        116     142
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     203     258 
   Size:        203      55
 
SmaI CCC'GGG

Cuts at:      0     249     258 
   Size:        249       9
 
XmaI C'CCGG_G

Cuts at:      0     247     258 
   Size:        247      11
 

 Enzymes that do cut:

   Acc65I     AcuI     AvaI     BaeI     BanI     BbsI     BmrI     BsaI
    BsrDI     EciI  Eco57MI    EcoRV    Hin4I     KpnI   MspA1I    PflMI
     SmaI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII    BamHI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI    EcoNI
 EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI