(Linear) (Six-base) MAPSORT of: KIEE6119.seq  Check: 5240  from: 1  to: 225



KIEE6119.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:24  ..

BclI T'GATC_A

Cuts at:      0     126     225 
   Size:        126      99
 
BsaHI Gr'CG_yC

Cuts at:      0      51     225 
   Size:         51     174
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     202     225 
   Size:        202      23
 
BsmI GAATG_Cn'

Cuts at:      0      76     225 
   Size:         76     149
 
BspHI T'CATG_A

Cuts at:      0     173     225 
   Size:        173      52
 
EaeI y'GGCC_r

Cuts at:      0      44     225 
   Size:         44     181
 
MscI TGG'CCA

Cuts at:      0      46     225 
   Size:         46     179
 
NdeI CA'TA_TG

Cuts at:      0      65     225 
   Size:         65     160
 
PasI CC'CwG_GG

Cuts at:      0      90     225 
   Size:         90     135
 
SfcI C'TryA_G

Cuts at:      0      71     156     225 
   Size:         71      85      69

  Fragments arranged by size: 

                  85      71      69
 

 Enzymes that do cut:

     BclI    BsaHI    BseRI     BsmI    BspHI     EaeI     MscI     NdeI
     PasI     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI