(Linear) (Six-base) MAPSORT of: KIEE6117.seq  Check: 2755  from: 1  to: 399



KIEE6117.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:24  ..

AatII G_ACGT'C

Cuts at:      0     127     399 
   Size:        127     272
 
ApaI G_GGCC'C

Cuts at:      0     288     330     399 
   Size:        288      42      69

  Fragments arranged by size: 

                 288      69      42
 
BanI G'GyrC_C

Cuts at:      0      99     399 
   Size:         99     300
 
BanII G_rGCy'C

Cuts at:      0     288     330     399 
   Size:        288      42      69

  Fragments arranged by size: 

                 288      69      42
 
BbeI G_GCGC'C

Cuts at:      0     103     399 
   Size:        103     296
 
Bme1580I G_kGCm'C

Cuts at:      0     164     288     330     399 
   Size:        164     124      42      69

  Fragments arranged by size: 

                 164     124      69      42
 
BmgBI CAC'GTC

Cuts at:      0     276     399 
   Size:        276     123
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     367     399 
   Size:        367      32
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     399 
   Size:         52     347
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     145     399 
   Size:        145     254
 
BsaBI GATnn'nnATC

Cuts at:      0     139     399 
   Size:        139     260
 
BsaHI Gr'CG_yC

Cuts at:      0     100     124     399 
   Size:        100      24     275

  Fragments arranged by size: 

                 275     100      24
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      88     399 
   Size:         88     311
 
BseYI C'CCAG_C

Cuts at:      0      93     399 
   Size:         93     306
 
BsiHKAI G_wGCw'C

Cuts at:      0      80     399 
   Size:         80     319
 
BsmI GAATG_Cn'

Cuts at:      0     229     399 
   Size:        229     170
 
Bsp1286I G_dGCh'C

Cuts at:      0      80     164     288     330     399 
   Size:         80      84     124      42      69

  Fragments arranged by size: 

                 124      84      80      69      42
 
BspHI T'CATG_A

Cuts at:      0     200     347     399 
   Size:        200     147      52

  Fragments arranged by size: 

                 200     147      52
 
BssSI C'ACGA_G

Cuts at:      0      79     399 
   Size:         79     320
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     394     399 
   Size:        394       5
 
DraIII CAC_nnn'GTG

Cuts at:      0      84     399 
   Size:         84     315
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     172     399 
   Size:        172     227
 
EaeI y'GGCC_r

Cuts at:      0      42     399 
   Size:         42     357
 
EarI CTCTTCn'nnn_

Cuts at:      0     202     399 
   Size:        202     197
 
EcoO109I rG'GnC_Cy

Cuts at:      0     284     327     399 
   Size:        284      43      72

  Fragments arranged by size: 

                 284      72      43
 
HaeII r_GCGC'y

Cuts at:      0     103     399 
   Size:        103     296
 
HincII GTy'rAC

Cuts at:      0      36     399 
   Size:         36     363
 
KasI G'GCGC_C

Cuts at:      0      99     399 
   Size:         99     300
 
MscI TGG'CCA

Cuts at:      0      44     399 
   Size:         44     355
 
MslI CAynn'nnrTG

Cuts at:      0     346     373     399 
   Size:        346      27      26

  Fragments arranged by size: 

                 346      27      26
 
NarI GG'CG_CC

Cuts at:      0     100     399 
   Size:        100     299
 
PspOMI G'GGCC_C

Cuts at:      0     284     326     399 
   Size:        284      42      73

  Fragments arranged by size: 

                 284      73      42
 
SfoI GGC'GCC

Cuts at:      0     101     399 
   Size:        101     298
 
SmlI C'TyrA_G

Cuts at:      0      67     399 
   Size:         67     332
 
StuI AGG'CCT

Cuts at:      0     292     391     399 
   Size:        292      99       8

  Fragments arranged by size: 

                 292      99       8
 
Tth111I GACn'n_nGTC

Cuts at:      0     169     399 
   Size:        169     230
 
ZraI GAC'GTC

Cuts at:      0     125     399 
   Size:        125     274
 

 Enzymes that do cut:

    AatII     ApaI     BanI    BanII     BbeI Bme1580I    BmgBI     BmrI
    BpuEI     BsaI    BsaBI    BsaHI    BseRI    BseYI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BssSI    BstXI   DraIII     DrdI     EaeI     EarI
 EcoO109I    HaeII   HincII     KasI     MscI     MslI     NarI   PspOMI
     SfoI     SmlI     StuI  Tth111I     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
     BmtI     BplI     BpmI   Bpu10I    BsaAI    BsaWI    BsaXI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI    TaqII
     TatI     TstI     XbaI     XcmI     XhoI     XmaI     XmnI