(Linear) (Six-base) MAPSORT of: KIEE6115.seq  Check: 2249  from: 1  to: 441



KIEE6115.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:24  ..

AccI GT'mk_AC

Cuts at:      0     104     441 
   Size:        104     337
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     217     441 
   Size:        217     224
 
ApoI r'AATT_y

Cuts at:      0     364     441 
   Size:        364      77
 
BamHI G'GATC_C

Cuts at:      0     297     441 
   Size:        297     144
 
BanI G'GyrC_C

Cuts at:      0     250     441 
   Size:        250     191
 
BglI GCCn_nnn'nGGC

Cuts at:      0      47     441 
   Size:         47     394
 
Bme1580I G_kGCm'C

Cuts at:      0     253     423     441 
   Size:        253     170      18

  Fragments arranged by size: 

                 253     170      18
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     414     441 
   Size:         92     322      27

  Fragments arranged by size: 

                 322      92      27
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     441 
   Size:        229     212
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      31      89     441 
   Size:         31      58     352

  Fragments arranged by size: 

                 352      58      31
 
BseYI C'CCAG_C

Cuts at:      0     260     441 
   Size:        260     181
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     400     441 
   Size:        259     141      41

  Fragments arranged by size: 

                 259     141      41
 
Bsp1286I G_dGCh'C

Cuts at:      0     253     423     441 
   Size:        253     170      18

  Fragments arranged by size: 

                 253     170      18
 
BstEII G'GTnAC_C

Cuts at:      0     333     441 
   Size:        333     108
 
BstYI r'GATC_y

Cuts at:      0     297     441 
   Size:        297     144
 
Bsu36I CC'TnA_GG

Cuts at:      0     374     441 
   Size:        374      67
 
BtgI C'CryG_G

Cuts at:      0     109     441 
   Size:        109     332
 
BtsI GCAGTG_nn'

Cuts at:      0     416     441 
   Size:        416      25
 
EaeI y'GGCC_r

Cuts at:      0     413     441 
   Size:        413      28
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     414     441 
   Size:         92     322      27

  Fragments arranged by size: 

                 322      92      27
 
EcoRI G'AATT_C

Cuts at:      0     364     441 
   Size:        364      77
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     270     441 
   Size:        270     171
 
MscI TGG'CCA

Cuts at:      0     415     441 
   Size:        415      26
 
MslI CAynn'nnrTG

Cuts at:      0     236     441 
   Size:        236     205
 
MspA1I CmG'CkG

Cuts at:      0     264     441 
   Size:        264     177
 
NcoI C'CATG_G

Cuts at:      0     109     441 
   Size:        109     332
 
NspI r_CATG'y

Cuts at:      0     295     441 
   Size:        295     146
 
PasI CC'CwG_GG

Cuts at:      0      84     441 
   Size:         84     357
 
PstI C_TGCA'G

Cuts at:      0     411     441 
   Size:        411      30
 
PvuII CAG'CTG

Cuts at:      0     264     441 
   Size:        264     177
 
SfcI C'TryA_G

Cuts at:      0     407     441 
   Size:        407      34
 
SmlI C'TyrA_G

Cuts at:      0     244     441 
   Size:        244     197
 
StyI C'CwwG_G

Cuts at:      0     109     212     441 
   Size:        109     103     229

  Fragments arranged by size: 

                 229     109     103
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     231     441 
   Size:        231     210
 

 Enzymes that do cut:

     AccI     AlfI     ApoI    BamHI     BanI     BglI Bme1580I     BpmI
    BpuEI    BseRI    BseYI     BsgI Bsp1286I   BstEII    BstYI   Bsu36I
     BtgI     BtsI     EaeI  Eco57MI    EcoRI     FalI     MscI     MslI
   MspA1I     NcoI     NspI     PasI     PstI    PvuII     SfcI     SmlI
     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI