Restriction map for
pFN21AE6114
(Linear) (Six-base) MAPSORT of: KIEE6114.seq Check: 6257 from: 1 to: 501
KIEE6114.orf
With 182 enzymes: SgfI *
December 5, 2014 13:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 501
Size: 322 179
AfeI AGC'GCT
Cuts at: 0 187 501
Size: 187 314
AlwNI CAG_nnn'CTG
Cuts at: 0 177 345 501
Size: 177 168 156
Fragments arranged by size:
177 168 156
AvaI C'yCGr_G
Cuts at: 0 100 501
Size: 100 401
BanI G'GyrC_C
Cuts at: 0 298 429 448 501
Size: 298 131 19 53
Fragments arranged by size:
298 131 53 19
BbeI G_GCGC'C
Cuts at: 0 302 501
Size: 302 199
BglI GCCn_nnn'nGGC
Cuts at: 0 64 501
Size: 64 437
BlpI GC'TnA_GC
Cuts at: 0 16 501
Size: 16 485
Bme1580I G_kGCm'C
Cuts at: 0 453 501
Size: 453 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 129 501
Size: 129 372
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 501
Size: 376 125
BsaI GGTCTCn'nnnn_
Cuts at: 0 139 501
Size: 139 362
BsaBI GATnn'nnATC
Cuts at: 0 214 501
Size: 214 287
BsaHI Gr'CG_yC
Cuts at: 0 299 501
Size: 299 202
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 118 139 325 501
Size: 49 69 21 186 176
Fragments arranged by size:
186 176 69 49 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 501
Size: 202 299
BsiEI CG_ry'CG
Cuts at: 0 225 501
Size: 225 276
Bsp1286I G_dGCh'C
Cuts at: 0 453 501
Size: 453 48
BsrBI CCG'CTC
Cuts at: 0 473 501
Size: 473 28
BstYI r'GATC_y
Cuts at: 0 78 501
Size: 78 423
BtgI C'CryG_G
Cuts at: 0 225 501
Size: 225 276
BtsI GCAGTG_nn'
Cuts at: 0 172 501
Size: 172 329
EaeI y'GGCC_r
Cuts at: 0 222 372 501
Size: 222 150 129
Fragments arranged by size:
222 150 129
EagI C'GGCC_G
Cuts at: 0 222 501
Size: 222 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 376 501
Size: 322 54 125
Fragments arranged by size:
322 125 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 121 501
Size: 121 380
HaeII r_GCGC'y
Cuts at: 0 51 189 302 355 501
Size: 51 138 113 53 146
Fragments arranged by size:
146 138 113 53 51
KasI G'GCGC_C
Cuts at: 0 298 501
Size: 298 203
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 501
Size: 11 490
MscI TGG'CCA
Cuts at: 0 374 501
Size: 374 127
MspA1I CmG'CkG
Cuts at: 0 246 258 270 417 501
Size: 246 12 12 147 84
Fragments arranged by size:
246 147 84 12 12
NarI GG'CG_CC
Cuts at: 0 299 501
Size: 299 202
PasI CC'CwG_GG
Cuts at: 0 366 501
Size: 366 135
PflMI CCAn_nnn'nTGG
Cuts at: 0 177 381 396 501
Size: 177 204 15 105
Fragments arranged by size:
204 177 105 15
PstI C_TGCA'G
Cuts at: 0 236 501
Size: 236 265
PvuII CAG'CTG
Cuts at: 0 246 417 501
Size: 246 171 84
Fragments arranged by size:
246 171 84
SfcI C'TryA_G
Cuts at: 0 232 501
Size: 232 269
SfoI GGC'GCC
Cuts at: 0 300 501
Size: 300 201
StuI AGG'CCT
Cuts at: 0 58 501
Size: 58 443
StyI C'CwwG_G
Cuts at: 0 305 501
Size: 305 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 148 358 501
Size: 148 210 143
Fragments arranged by size:
210 148 143
Tth111I GACn'n_nGTC
Cuts at: 0 87 501
Size: 87 414
Enzymes that do cut:
AcuI AfeI AlwNI AvaI BanI BbeI BglI BlpI
Bme1580I BplI BpmI BsaI BsaBI BsaHI BseRI BsgI
BsiEI Bsp1286I BsrBI BstYI BtgI BtsI EaeI EagI
Eco57MI EcoNI HaeII KasI MmeI MscI MspA1I NarI
PasI PflMI PstI PvuII SfcI SfoI StuI StyI
TaqII Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI
Bpu10I BpuEI BsaAI BsaWI BsaXI BseYI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EarI EciI
EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI XbaI XcmI XhoI XmaI
XmnI ZraI