(Linear) (Six-base) MAPSORT of: KIEE6112.seq  Check: 4793  from: 1  to: 405



KIEE6112.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:24  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      18     405 
   Size:         18     387
 
BanI G'GyrC_C

Cuts at:      0      32     405 
   Size:         32     373
 
BbeI G_GCGC'C

Cuts at:      0      36     405 
   Size:         36     369
 
BsaHI Gr'CG_yC

Cuts at:      0      33     405 
   Size:         33     372
 
BsiHKAI G_wGCw'C

Cuts at:      0     175     405 
   Size:        175     230
 
Bsp1286I G_dGCh'C

Cuts at:      0     175     405 
   Size:        175     230
 
BspHI T'CATG_A

Cuts at:      0     362     405 
   Size:        362      43
 
BsrDI GCAATG_nn'

Cuts at:      0     116     405 
   Size:        116     289
 
BstYI r'GATC_y

Cuts at:      0     386     405 
   Size:        386      19
 
EaeI y'GGCC_r

Cuts at:      0       2     113     405 
   Size:          2     111     292

  Fragments arranged by size: 

                 292     111       2
 
HaeII r_GCGC'y

Cuts at:      0      36     405 
   Size:         36     369
 
KasI G'GCGC_C

Cuts at:      0      32     405 
   Size:         32     373
 
MscI TGG'CCA

Cuts at:      0       4     115     405 
   Size:          4     111     290

  Fragments arranged by size: 

                 290     111       4
 
MspA1I CmG'CkG

Cuts at:      0      18     232     405 
   Size:         18     214     173

  Fragments arranged by size: 

                 214     173      18
 
NarI GG'CG_CC

Cuts at:      0      33     405 
   Size:         33     372
 
PasI CC'CwG_GG

Cuts at:      0     204     405 
   Size:        204     201
 
PvuII CAG'CTG

Cuts at:      0      18     232     405 
   Size:         18     214     173

  Fragments arranged by size: 

                 214     173      18
 
SfoI GGC'GCC

Cuts at:      0      34     405 
   Size:         34     371
 
TatI w'GTAC_w

Cuts at:      0     379     405 
   Size:        379      26
 

 Enzymes that do cut:

    AlwNI     BanI     BbeI    BsaHI  BsiHKAI Bsp1286I    BspHI    BsrDI
    BstYI     EaeI    HaeII     KasI     MscI   MspA1I     NarI     PasI
    PvuII     SfoI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MslI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI