Restriction map for
pFN21AE6111
(Linear) (Six-base) MAPSORT of: KIEE6111.seq Check: 4153 from: 1 to: 426
KIEE6111.orf
With 182 enzymes: SgfI *
December 5, 2014 13:24 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 279 426
Size: 279 147
AlwNI CAG_nnn'CTG
Cuts at: 0 24 426
Size: 24 402
ApaI G_GGCC'C
Cuts at: 0 312 426
Size: 312 114
AvaI C'yCGr_G
Cuts at: 0 52 312 426
Size: 52 260 114
Fragments arranged by size:
260 114 52
BanI G'GyrC_C
Cuts at: 0 319 426
Size: 319 107
BanII G_rGCy'C
Cuts at: 0 312 355 426
Size: 312 43 71
Fragments arranged by size:
312 71 43
BbeI G_GCGC'C
Cuts at: 0 323 426
Size: 323 103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 102 136 426
Size: 102 34 290
Fragments arranged by size:
290 102 34
BglI GCCn_nnn'nGGC
Cuts at: 0 217 426
Size: 217 209
BlpI GC'TnA_GC
Cuts at: 0 416 426
Size: 416 10
Bme1580I G_kGCm'C
Cuts at: 0 312 426
Size: 312 114
BsaHI Gr'CG_yC
Cuts at: 0 173 320 335 426
Size: 173 147 15 91
Fragments arranged by size:
173 147 91 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 232 262 426
Size: 232 30 164
Fragments arranged by size:
232 164 30
BseYI C'CCAG_C
Cuts at: 0 103 426
Size: 103 323
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 426
Size: 43 383
BsiHKAI G_wGCw'C
Cuts at: 0 302 426
Size: 302 124
Bsp1286I G_dGCh'C
Cuts at: 0 302 312 355 426
Size: 302 10 43 71
Fragments arranged by size:
302 71 43 10
BspHI T'CATG_A
Cuts at: 0 197 426
Size: 197 229
BsrBI CCG'CTC
Cuts at: 0 248 420 426
Size: 248 172 6
Fragments arranged by size:
248 172 6
BstEII G'GTnAC_C
Cuts at: 0 150 426
Size: 150 276
BstXI CCAn_nnnn'nTGG
Cuts at: 0 84 426
Size: 84 342
BtgI C'CryG_G
Cuts at: 0 347 426
Size: 347 79
EaeI y'GGCC_r
Cuts at: 0 218 426
Size: 218 208
EcoNI CCTnn'n_nnAGG
Cuts at: 0 406 426
Size: 406 20
EcoO109I rG'GnC_Cy
Cuts at: 0 309 426
Size: 309 117
HaeII r_GCGC'y
Cuts at: 0 92 161 323 416 426
Size: 92 69 162 93 10
Fragments arranged by size:
162 93 92 69 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 215 247 265 297 426
Size: 215 32 18 32 129
Fragments arranged by size:
215 129 32 32 18
KasI G'GCGC_C
Cuts at: 0 319 426
Size: 319 107
MslI CAynn'nnrTG
Cuts at: 0 112 426
Size: 112 314
MspA1I CmG'CkG
Cuts at: 0 21 80 349 426
Size: 21 59 269 77
Fragments arranged by size:
269 77 59 21
NarI GG'CG_CC
Cuts at: 0 320 426
Size: 320 106
PasI CC'CwG_GG
Cuts at: 0 408 426
Size: 408 18
PspOMI G'GGCC_C
Cuts at: 0 308 426
Size: 308 118
PspXI vC'TCGA_Gb
Cuts at: 0 52 426
Size: 52 374
PstI C_TGCA'G
Cuts at: 0 10 426
Size: 10 416
SacII CC_GC'GG
Cuts at: 0 350 426
Size: 350 76
SfcI C'TryA_G
Cuts at: 0 6 426
Size: 6 420
SfoI GGC'GCC
Cuts at: 0 321 426
Size: 321 105
SmaI CCC'GGG
Cuts at: 0 314 426
Size: 314 112
SmlI C'TyrA_G
Cuts at: 0 52 426
Size: 52 374
StuI AGG'CCT
Cuts at: 0 227 426
Size: 227 199
StyI C'CwwG_G
Cuts at: 0 161 269 426
Size: 161 108 157
Fragments arranged by size:
161 157 108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 111 426
Size: 111 315
XhoI C'TCGA_G
Cuts at: 0 52 426
Size: 52 374
XmaI C'CCGG_G
Cuts at: 0 312 426
Size: 312 114
Enzymes that do cut:
AhdI AlwNI ApaI AvaI BanI BanII BbeI BcgI
BglI BlpI Bme1580I BsaHI BsaXI BseYI BsgI BsiHKAI
Bsp1286I BspHI BsrBI BstEII BstXI BtgI EaeI EcoNI
EcoO109I HaeII Hin4I KasI MslI MspA1I NarI PasI
PspOMI PspXI PstI SacII SfcI SfoI SmaI SmlI
StuI StyI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI
BclI BfrBI BglII BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI Eco57MI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PsrI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TatI TstI Tth111I XbaI XmnI ZraI