(Linear) (Six-base) MAPSORT of: KIEE6108.seq  Check: 3967  from: 1  to: 363



KIEE6108.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:24  ..

AvaI C'yCGr_G

Cuts at:      0     109     363 
   Size:        109     254
 
BanI G'GyrC_C

Cuts at:      0     157     363 
   Size:        157     206
 
BbeI G_GCGC'C

Cuts at:      0     161     363 
   Size:        161     202
 
BglI GCCn_nnn'nGGC

Cuts at:      0      39     363 
   Size:         39     324
 
Bme1580I G_kGCm'C

Cuts at:      0     169     363 
   Size:        169     194
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     348     363 
   Size:        348      15
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     363 
   Size:        205     158
 
Bpu10I CC'TnA_GC

Cuts at:      0     293     363 
   Size:        293      70
 
BsaHI Gr'CG_yC

Cuts at:      0     158     363 
   Size:        158     205
 
BsiEI CG_ry'CG

Cuts at:      0      73     363 
   Size:         73     290
 
Bsp1286I G_dGCh'C

Cuts at:      0     169     363 
   Size:        169     194
 
BsrDI GCAATG_nn'

Cuts at:      0     357     363 
   Size:        357       6
 
BsrFI r'CCGG_y

Cuts at:      0      68     363 
   Size:         68     295
 
BssHII G'CGCG_C

Cuts at:      0      91     363 
   Size:         91     272
 
BstYI r'GATC_y

Cuts at:      0     342     363 
   Size:        342      21
 
EaeI y'GGCC_r

Cuts at:      0      70     143     363 
   Size:         70      73     220

  Fragments arranged by size: 

                 220      73      70
 
EagI C'GGCC_G

Cuts at:      0      70     363 
   Size:         70     293
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     144     363 
   Size:        144     219
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     363 
   Size:        205     158
 
EcoO109I rG'GnC_Cy

Cuts at:      0     272     363 
   Size:        272      91
 
HaeII r_GCGC'y

Cuts at:      0     161     363 
   Size:        161     202
 
KasI G'GCGC_C

Cuts at:      0     157     363 
   Size:        157     206
 
MspA1I CmG'CkG

Cuts at:      0     149     363 
   Size:        149     214
 
NaeI GCC'GGC

Cuts at:      0      70     363 
   Size:         70     293
 
NarI GG'CG_CC

Cuts at:      0     158     363 
   Size:        158     205
 
NgoMIV G'CCGG_C

Cuts at:      0      68     363 
   Size:         68     295
 
NspI r_CATG'y

Cuts at:      0      99     363 
   Size:         99     264
 
PstI C_TGCA'G

Cuts at:      0     243     363 
   Size:        243     120
 
RsrII CG'GwC_CG

Cuts at:      0     151     363 
   Size:        151     212
 
SfcI C'TryA_G

Cuts at:      0     239     363 
   Size:        239     124
 
SfoI GGC'GCC

Cuts at:      0     159     363 
   Size:        159     204
 
SmaI CCC'GGG

Cuts at:      0     111     363 
   Size:        111     252
 
SphI G_CATG'C

Cuts at:      0      99     363 
   Size:         99     264
 
SrfI GCCC'GGGC

Cuts at:      0     111     363 
   Size:        111     252
 
TatI w'GTAC_w

Cuts at:      0     325     363 
   Size:        325      38
 
XmaI C'CCGG_G

Cuts at:      0     109     363 
   Size:        109     254
 

 Enzymes that do cut:

     AvaI     BanI     BbeI     BglI Bme1580I     BmrI     BpmI   Bpu10I
    BsaHI    BsiEI Bsp1286I    BsrDI    BsrFI   BssHII    BstYI     EaeI
     EagI     EciI  Eco57MI EcoO109I    HaeII     KasI   MspA1I     NaeI
     NarI   NgoMIV     NspI     PstI    RsrII     SfcI     SfoI     SmaI
     SphI     SrfI     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BanII     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrGI    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EarI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI