(Linear) (Six-base) MAPSORT of: KIEE6107.seq  Check: 6970  from: 1  to: 471



KIEE6107.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     471 
   Size:        181     290
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     347     379     471 
   Size:        347      32      92

  Fragments arranged by size: 

                 347      92      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     290     471 
   Size:        290     181
 
BglII A'GATC_T

Cuts at:      0     156     471 
   Size:        156     315
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     471 
   Size:        166     305
 
BsaAI yAC'GTr

Cuts at:      0     442     471 
   Size:        442      29
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     347     377     471 
   Size:        347      30      94

  Fragments arranged by size: 

                 347      94      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     156     471 
   Size:        156     315
 
BstYI r'GATC_y

Cuts at:      0     156     471 
   Size:        156     315
 
BtsI GCAGTG_nn'

Cuts at:      0     291     471 
   Size:        291     180
 
DraIII CAC_nnn'GTG

Cuts at:      0     445     471 
   Size:        445      26
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     471 
   Size:        181     290
 
EcoRV GAT'ATC

Cuts at:      0     303     471 
   Size:        303     168
 
HindIII A'AGCT_T

Cuts at:      0      34     471 
   Size:         34     437
 
MfeI C'AATT_G

Cuts at:      0     398     471 
   Size:        398      73
 
MslI CAynn'nnrTG

Cuts at:      0     254     471 
   Size:        254     217
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      86     118     347     379     471 
   Size:         86      32     229      32      92

  Fragments arranged by size: 

                 229      92      86      32      32
 
PstI C_TGCA'G

Cuts at:      0     422     471 
   Size:        422      49
 
SfcI C'TryA_G

Cuts at:      0     418     471 
   Size:        418      53
 
SmlI C'TyrA_G

Cuts at:      0     145     471 
   Size:        145     326
 
XmnI GAAnn'nnTTC

Cuts at:      0     101     245     471 
   Size:        101     144     226

  Fragments arranged by size: 

                 226     144     101
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI    BglII    BpuEI    BsaAI    BsaXI     BsgI
    BstYI     BtsI   DraIII  Eco57MI    EcoRV  HindIII     MfeI     MslI
     PpiI     PstI     SfcI     SmlI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
     BsaI    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
     HpaI     KasI     KpnI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI