(Linear) (Six-base) MAPSORT of: KIEE6105.seq  Check: 4151  from: 1  to: 246



KIEE6105.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:23  ..

BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     246 
   Size:         67     179
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      85     246 
   Size:         85     161
 
BseYI C'CCAG_C

Cuts at:      0      95     246 
   Size:         95     151
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     246 
   Size:         49     197
 
BssSI C'ACGA_G

Cuts at:      0      67     246 
   Size:         67     179
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     115     246 
   Size:        115     131
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      39     246 
   Size:         39     207
 
BtsI GCAGTG_nn'

Cuts at:      0      19      65     246 
   Size:         19      46     181

  Fragments arranged by size: 

                 181      46      19
 
EarI CTCTTCn'nnn_

Cuts at:      0     125     246 
   Size:        125     121
 
HincII GTy'rAC

Cuts at:      0      48     246 
   Size:         48     198
 
MfeI C'AATT_G

Cuts at:      0     215     221     246 
   Size:        215       6      25

  Fragments arranged by size: 

                 215      25       6
 
MslI CAynn'nnrTG

Cuts at:      0      58     246 
   Size:         58     188
 
PstI C_TGCA'G

Cuts at:      0      29     246 
   Size:         29     217
 
SapI GCTCTTCn'nnn_

Cuts at:      0     125     246 
   Size:        125     121
 
SfcI C'TryA_G

Cuts at:      0      25     246 
   Size:         25     221
 
SmlI C'TyrA_G

Cuts at:      0      82     246 
   Size:         82     164
 
TatI w'GTAC_w

Cuts at:      0      14     246 
   Size:         14     232
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     115     246 
   Size:        115     131
 

 Enzymes that do cut:

    BpuEI    BseRI    BseYI     BsgI    BssSI    BstXI    BtgZI     BtsI
     EarI   HincII     MfeI     MslI     PstI     SapI     SfcI     SmlI
     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI