Restriction map for
pFN21AE6096
(Linear) (Six-base) MAPSORT of: KIEE6096.seq Check: 5377 from: 1 to: 978
KIEE6096.orf
With 182 enzymes: SgfI *
December 5, 2014 13:23 ..
Acc65I G'GTAC_C
Cuts at: 0 673 978
Size: 673 305
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 719 978
Size: 94 625 259
Fragments arranged by size:
625 259 94
AflIII A'CryG_T
Cuts at: 0 111 978
Size: 111 867
AlwNI CAG_nnn'CTG
Cuts at: 0 526 661 978
Size: 526 135 317
Fragments arranged by size:
526 317 135
ApaLI G'TGCA_C
Cuts at: 0 239 978
Size: 239 739
ApoI r'AATT_y
Cuts at: 0 32 79 634 978
Size: 32 47 555 344
Fragments arranged by size:
555 344 47 32
BamHI G'GATC_C
Cuts at: 0 728 978
Size: 728 250
BanI G'GyrC_C
Cuts at: 0 673 978
Size: 673 305
BbsI GAAGACnn'nnnn_
Cuts at: 0 129 696 978
Size: 129 567 282
Fragments arranged by size:
567 282 129
BbvCI CC'TCA_GC
Cuts at: 0 740 892 978
Size: 740 152 86
Fragments arranged by size:
740 152 86
BclI T'GATC_A
Cuts at: 0 176 978
Size: 176 802
BglII A'GATC_T
Cuts at: 0 297 978
Size: 297 681
Bme1580I G_kGCm'C
Cuts at: 0 243 978
Size: 243 735
Bpu10I CC'TnA_GC
Cuts at: 0 740 892 978
Size: 740 152 86
Fragments arranged by size:
740 152 86
BsaI GGTCTCn'nnnn_
Cuts at: 0 250 978
Size: 250 728
BsaBI GATnn'nnATC
Cuts at: 0 55 978
Size: 55 923
BseYI C'CCAG_C
Cuts at: 0 669 978
Size: 669 309
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 978
Size: 512 466
BsiHKAI G_wGCw'C
Cuts at: 0 243 978
Size: 243 735
Bsp1286I G_dGCh'C
Cuts at: 0 243 978
Size: 243 735
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 157 268 978
Size: 157 111 710
Fragments arranged by size:
710 157 111
BsrGI T'GTAC_A
Cuts at: 0 114 978
Size: 114 864
BstXI CCAn_nnnn'nTGG
Cuts at: 0 291 978
Size: 291 687
BstYI r'GATC_y
Cuts at: 0 297 728 978
Size: 297 431 250
Fragments arranged by size:
431 297 250
Bsu36I CC'TnA_GG
Cuts at: 0 724 840 913 978
Size: 724 116 73 65
Fragments arranged by size:
724 116 73 65
BtgI C'CryG_G
Cuts at: 0 785 978
Size: 785 193
BtsI GCAGTG_nn'
Cuts at: 0 445 494 978
Size: 445 49 484
Fragments arranged by size:
484 445 49
EaeI y'GGCC_r
Cuts at: 0 305 419 978
Size: 305 114 559
Fragments arranged by size:
559 305 114
EarI CTCTTCn'nnn_
Cuts at: 0 235 978
Size: 235 743
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 719 978
Size: 94 625 259
Fragments arranged by size:
625 259 94
EcoO109I rG'GnC_Cy
Cuts at: 0 206 978
Size: 206 772
EcoRI G'AATT_C
Cuts at: 0 79 634 978
Size: 79 555 344
Fragments arranged by size:
555 344 79
EcoRV GAT'ATC
Cuts at: 0 442 978
Size: 442 536
KpnI G_GTAC'C
Cuts at: 0 677 978
Size: 677 301
MscI TGG'CCA
Cuts at: 0 307 421 978
Size: 307 114 557
Fragments arranged by size:
557 307 114
MslI CAynn'nnrTG
Cuts at: 0 220 397 978
Size: 220 177 581
Fragments arranged by size:
581 220 177
MspA1I CmG'CkG
Cuts at: 0 739 978
Size: 739 239
NdeI CA'TA_TG
Cuts at: 0 587 978
Size: 587 391
NspI r_CATG'y
Cuts at: 0 115 978
Size: 115 863
PciI A'CATG_T
Cuts at: 0 111 978
Size: 111 867
PflMI CCAn_nnn'nTGG
Cuts at: 0 591 978
Size: 591 387
PpuMI rG'GwC_Cy
Cuts at: 0 206 978
Size: 206 772
PshAI GACnn'nnGTC
Cuts at: 0 277 700 978
Size: 277 423 278
Fragments arranged by size:
423 278 277
PsiI TTA'TAA
Cuts at: 0 643 793 978
Size: 643 150 185
Fragments arranged by size:
643 185 150
PstI C_TGCA'G
Cuts at: 0 501 556 978
Size: 501 55 422
Fragments arranged by size:
501 422 55
PvuII CAG'CTG
Cuts at: 0 739 978
Size: 739 239
SfcI C'TryA_G
Cuts at: 0 497 552 978
Size: 497 55 426
Fragments arranged by size:
497 426 55
StuI AGG'CCT
Cuts at: 0 757 978
Size: 757 221
StyI C'CwwG_G
Cuts at: 0 308 978
Size: 308 670
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 186 978
Size: 186 792
TatI w'GTAC_w
Cuts at: 0 114 978
Size: 114 864
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 464 496 978
Size: 464 32 482
Fragments arranged by size:
482 464 32
XmnI GAAnn'nnTTC
Cuts at: 0 79 83 321 757 978
Size: 79 4 238 436 221
Fragments arranged by size:
436 238 221 79 4
Enzymes that do cut:
Acc65I AcuI AflIII AlwNI ApaLI ApoI BamHI BanI
BbsI BbvCI BclI BglII Bme1580I Bpu10I BsaI BsaBI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrGI BstXI BstYI
Bsu36I BtgI BtsI EaeI EarI Eco57MI EcoO109I EcoRI
EcoRV KpnI MscI MslI MspA1I NdeI NspI PciI
PflMI PpuMI PshAI PsiI PstI PvuII SfcI StuI
StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BanII BbeI BcgI BciVI BfrBI BglI
BlpI BmgBI BmrI BmtI BplI BpmI BpuEI BsaAI
BsaHI BsaWI BsaXI BseRI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI MfeI
MluI MmeI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PasI PfoI PmeI PmlI PpiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XcmI XhoI XmaI ZraI