(Linear) (Six-base) MAPSORT of: KIEE6095.seq  Check: 6639  from: 1  to: 978



KIEE6095.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:23  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     733     749     978 
   Size:         77     656      16     229

  Fragments arranged by size: 

                 656     229      77      16
 
AflIII A'CryG_T

Cuts at:      0     754     978 
   Size:        754     224
 
ApoI r'AATT_y

Cuts at:      0      94     978 
   Size:         94     884
 
AseI AT'TA_AT

Cuts at:      0      76     978 
   Size:         76     902
 
BamHI G'GATC_C

Cuts at:      0     544     978 
   Size:        544     434
 
BanII G_rGCy'C

Cuts at:      0     649     765     978 
   Size:        649     116     213

  Fragments arranged by size: 

                 649     213     116
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     102     738     978 
   Size:        102     636     240

  Fragments arranged by size: 

                 636     240     102
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     794     978 
   Size:        794     184
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     810     978 
   Size:        810     168
 
BsaAI yAC'GTr

Cuts at:      0     615     978 
   Size:        615     363
 
BsaBI GATnn'nnATC

Cuts at:      0      23     978 
   Size:         23     955
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     572     750     978 
   Size:        572     178     228

  Fragments arranged by size: 

                 572     228     178
 
BsiHKAI G_wGCw'C

Cuts at:      0     765     978 
   Size:        765     213
 
BsmI GAATG_Cn'

Cuts at:      0     373     978 
   Size:        373     605
 
Bsp1286I G_dGCh'C

Cuts at:      0     649     765     978 
   Size:        649     116     213

  Fragments arranged by size: 

                 649     213     116
 
BsrDI GCAATG_nn'

Cuts at:      0     392     978 
   Size:        392     586
 
BstYI r'GATC_y

Cuts at:      0      24     544     978 
   Size:         24     520     434

  Fragments arranged by size: 

                 520     434      24
 
BtsI GCAGTG_nn'

Cuts at:      0     470     978 
   Size:        470     508
 
EaeI y'GGCC_r

Cuts at:      0     704     978 
   Size:        704     274
 
EarI CTCTTCn'nnn_

Cuts at:      0     808     978 
   Size:        808     170
 
EcoICRI GAG'CTC

Cuts at:      0     763     978 
   Size:        763     215
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     733     749     794     978 
   Size:         77     656      16      45     184

  Fragments arranged by size: 

                 656     184      77      45      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     771     803     978 
   Size:        771      32     175

  Fragments arranged by size: 

                 771     175      32
 
HindIII A'AGCT_T

Cuts at:      0      34      81     978 
   Size:         34      47     897

  Fragments arranged by size: 

                 897      47      34
 
MscI TGG'CCA

Cuts at:      0     706     978 
   Size:        706     272
 
MslI CAynn'nnrTG

Cuts at:      0     811     853     978 
   Size:        811      42     125

  Fragments arranged by size: 

                 811     125      42
 
NspI r_CATG'y

Cuts at:      0     444     502     565     758     978 
   Size:        444      58      63     193     220

  Fragments arranged by size: 

                 444     220     193      63      58
 
PciI A'CATG_T

Cuts at:      0     754     978 
   Size:        754     224
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     807     839     978 
   Size:        807      32     139

  Fragments arranged by size: 

                 807     139      32
 
SacI G_AGCT'C

Cuts at:      0     765     978 
   Size:        765     213
 
SfcI C'TryA_G

Cuts at:      0     257     903     978 
   Size:        257     646      75

  Fragments arranged by size: 

                 646     257      75
 
SspI AAT'ATT

Cuts at:      0     863     978 
   Size:        863     115
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     462     978 
   Size:        462     516
 
TatI w'GTAC_w

Cuts at:      0     212     349     978 
   Size:        212     137     629

  Fragments arranged by size: 

                 629     212     137
 
XmnI GAAnn'nnTTC

Cuts at:      0     371     978 
   Size:        371     607
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     AseI    BamHI    BanII     BbsI     BpmI
     BsaI    BsaAI    BsaBI    BseRI  BsiHKAI     BsmI Bsp1286I    BsrDI
    BstYI     BtsI     EaeI     EarI  EcoICRI  Eco57MI    Hin4I  HindIII
     MscI     MslI     NspI     PciI     PpiI     SacI     SfcI     SspI
    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI