Restriction map for
pFN21AE6094
(Linear) (Six-base) MAPSORT of: KIEE6094.seq Check: 3981 from: 1 to: 978
KIEE6094.orf
With 182 enzymes: SgfI *
December 5, 2014 13:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 478 775 978
Size: 478 297 203
Fragments arranged by size:
478 297 203
AccI GT'mk_AC
Cuts at: 0 494 978
Size: 494 484
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 744 978
Size: 744 234
AleI CACnn'nnGTG
Cuts at: 0 166 978
Size: 166 812
AlwNI CAG_nnn'CTG
Cuts at: 0 767 978
Size: 767 211
AvaI C'yCGr_G
Cuts at: 0 145 978
Size: 145 833
BbsI GAAGACnn'nnnn_
Cuts at: 0 636 978
Size: 636 342
BbvCI CC'TCA_GC
Cuts at: 0 961 978
Size: 961 17
BclI T'GATC_A
Cuts at: 0 185 978
Size: 185 793
BfrBI ATG'CAT
Cuts at: 0 21 978
Size: 21 957
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 133 978
Size: 133 845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 699 978
Size: 103 596 279
Fragments arranged by size:
596 279 103
Bpu10I CC'TnA_GC
Cuts at: 0 961 978
Size: 961 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 978
Size: 76 902
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 310 340 978
Size: 310 30 638
Fragments arranged by size:
638 310 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 60 127 261 978
Size: 60 67 134 717
Fragments arranged by size:
717 134 67 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 970 978
Size: 199 771 8
Fragments arranged by size:
771 199 8
BsiEI CG_ry'CG
Cuts at: 0 414 978
Size: 414 564
BsiHKAI G_wGCw'C
Cuts at: 0 563 978
Size: 563 415
BsmI GAATG_Cn'
Cuts at: 0 814 978
Size: 814 164
Bsp1286I G_dGCh'C
Cuts at: 0 563 978
Size: 563 415
BspHI T'CATG_A
Cuts at: 0 599 978
Size: 599 379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 478 775 978
Size: 478 297 203
Fragments arranged by size:
478 297 203
BsrDI GCAATG_nn'
Cuts at: 0 374 978
Size: 374 604
BssSI C'ACGA_G
Cuts at: 0 675 978
Size: 675 303
BstEII G'GTnAC_C
Cuts at: 0 159 978
Size: 159 819
BstYI r'GATC_y
Cuts at: 0 149 258 748 978
Size: 149 109 490 230
Fragments arranged by size:
490 230 149 109
Bsu36I CC'TnA_GG
Cuts at: 0 922 978
Size: 922 56
BtgI C'CryG_G
Cuts at: 0 788 884 978
Size: 788 96 94
Fragments arranged by size:
788 96 94
EarI CTCTTCn'nnn_
Cuts at: 0 100 145 978
Size: 100 45 833
Fragments arranged by size:
833 100 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 699 744 978
Size: 103 596 45 234
Fragments arranged by size:
596 234 103 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 650 682 733 765 978
Size: 650 32 51 32 213
Fragments arranged by size:
650 213 51 32 32
MslI CAynn'nnrTG
Cuts at: 0 166 223 454 978
Size: 166 57 231 524
Fragments arranged by size:
524 231 166 57
MspA1I CmG'CkG
Cuts at: 0 213 978
Size: 213 765
NsiI A_TGCA'T
Cuts at: 0 23 978
Size: 23 955
NspI r_CATG'y
Cuts at: 0 345 393 978
Size: 345 48 585
Fragments arranged by size:
585 345 48
PstI C_TGCA'G
Cuts at: 0 31 60 772 978
Size: 31 29 712 206
Fragments arranged by size:
712 206 31 29
PvuII CAG'CTG
Cuts at: 0 213 978
Size: 213 765
SbfI CC_TGCA'GG
Cuts at: 0 31 978
Size: 31 947
SfcI C'TryA_G
Cuts at: 0 27 56 71 768 915 978
Size: 27 29 15 697 147 63
Fragments arranged by size:
697 147 63 29 27 15
SmlI C'TyrA_G
Cuts at: 0 91 978
Size: 91 887
StuI AGG'CCT
Cuts at: 0 33 978
Size: 33 945
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 55 978
Size: 55 923
TatI w'GTAC_w
Cuts at: 0 227 821 978
Size: 227 594 157
Fragments arranged by size:
594 227 157
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI AvaI BbsI BbvCI
BclI BfrBI BplI BpmI Bpu10I BpuEI BsaXI BseRI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BssSI BstEII BstYI Bsu36I BtgI EarI Eco57MI Hin4I
MslI MspA1I NsiI NspI PstI PvuII SbfI SfcI
SmlI StuI TaqII TatI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BanII BbeI BcgI BciVI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiWI BsmBI BspEI
BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstXI BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI