(Linear) (Six-base) MAPSORT of: KIEE6092.seq  Check: 895  from: 1  to: 978



KIEE6092.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:23  ..

AflIII A'CryG_T

Cuts at:      0     224     978 
   Size:        224     754
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     482     978 
   Size:        482     496
 
ApoI r'AATT_y

Cuts at:      0      51      76     237     978 
   Size:         51      25     161     741

  Fragments arranged by size: 

                 741     161      51      25
 
BamHI G'GATC_C

Cuts at:      0     691     978 
   Size:        691     287
 
BanII G_rGCy'C

Cuts at:      0     829     978 
   Size:        829     149
 
BclI T'GATC_A

Cuts at:      0     405     978 
   Size:        405     573
 
BglII A'GATC_T

Cuts at:      0     116     978 
   Size:        116     862
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     464     749     978 
   Size:        464     285     229

  Fragments arranged by size: 

                 464     285     229
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325     904     978 
   Size:        325     579      74

  Fragments arranged by size: 

                 579     325      74
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     776     978 
   Size:        776     202
 
BseYI C'CCAG_C

Cuts at:      0     488     978 
   Size:        488     490
 
BsmI GAATG_Cn'

Cuts at:      0     382     978 
   Size:        382     596
 
Bsp1286I G_dGCh'C

Cuts at:      0     829     978 
   Size:        829     149
 
BspHI T'CATG_A

Cuts at:      0     449     978 
   Size:        449     529
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0       9     978 
   Size:          9     969
 
BsrDI GCAATG_nn'

Cuts at:      0     176     978 
   Size:        176     802
 
BsrGI T'GTAC_A

Cuts at:      0     221     978 
   Size:        221     757
 
BstEII G'GTnAC_C

Cuts at:      0     885     978 
   Size:        885      93
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     288     978 
   Size:        288     690
 
BstYI r'GATC_y

Cuts at:      0     116     691     978 
   Size:        116     575     287

  Fragments arranged by size: 

                 575     287     116
 
Bsu36I CC'TnA_GG

Cuts at:      0     946     978 
   Size:        946      32
 
EarI CTCTTCn'nnn_

Cuts at:      0     130     273     901     978 
   Size:        130     143     628      77

  Fragments arranged by size: 

                 628     143     130      77
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325     904     978 
   Size:        325     579      74

  Fragments arranged by size: 

                 579     325      74
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     323     355     444     476     978 
   Size:        323      32      89      32     502

  Fragments arranged by size: 

                 502     323      89      32      32
 
HincII GTy'rAC

Cuts at:      0     294     978 
   Size:        294     684
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     654     978 
   Size:        654     324
 
MslI CAynn'nnrTG

Cuts at:      0     217     223     375     464     820     978 
   Size:        217       6     152      89     356     158

  Fragments arranged by size: 

                 356     217     158     152      89       6
 
NdeI CA'TA_TG

Cuts at:      0     147     978 
   Size:        147     831
 
NspI r_CATG'y

Cuts at:      0     228     978 
   Size:        228     750
 
PasI CC'CwG_GG

Cuts at:      0     586     978 
   Size:        586     392
 
PciI A'CATG_T

Cuts at:      0     224     978 
   Size:        224     754
 
SfcI C'TryA_G

Cuts at:      0     272     798     845     912     978 
   Size:        272     526      47      67      66

  Fragments arranged by size: 

                 526     272      67      66      47
 
StuI AGG'CCT

Cuts at:      0     754     978 
   Size:        754     224
 
StyI C'CwwG_G

Cuts at:      0      87     978 
   Size:         87     891
 
TatI w'GTAC_w

Cuts at:      0     221     978 
   Size:        221     757
 

 Enzymes that do cut:

   AflIII     AlfI     ApoI    BamHI    BanII     BclI    BglII     BmrI
     BpmI     BsaI    BseYI     BsmI Bsp1286I    BspHI    BspMI    BsrDI
    BsrGI   BstEII    BstXI    BstYI   Bsu36I     EarI  Eco57MI    Hin4I
   HincII     MmeI     MslI     NdeI     NspI     PasI     PciI     SfcI
     StuI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI
    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI