(Linear) (Six-base) MAPSORT of: KIEE6091.seq  Check: 5586  from: 1  to: 966



KIEE6091.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:23  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     652     966 
   Size:        652     314
 
BanII G_rGCy'C

Cuts at:      0     768     823     966 
   Size:        768      55     143

  Fragments arranged by size: 

                 768     143      55
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     731     966 
   Size:        731     235
 
BclI T'GATC_A

Cuts at:      0     293     966 
   Size:        293     673
 
BfrBI ATG'CAT

Cuts at:      0     338     461     966 
   Size:        338     123     505

  Fragments arranged by size: 

                 505     338     123
 
BsaBI GATnn'nnATC

Cuts at:      0     292     966 
   Size:        292     674
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     264     294     966 
   Size:        264      30     672

  Fragments arranged by size: 

                 672     264      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58     966 
   Size:         58     908
 
BsmI GAATG_Cn'

Cuts at:      0     136     340     461     966 
   Size:        136     204     121     505

  Fragments arranged by size: 

                 505     204     136     121
 
Bsp1286I G_dGCh'C

Cuts at:      0     768     823     966 
   Size:        768      55     143

  Fragments arranged by size: 

                 768     143      55
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     637     966 
   Size:        637     329
 
BsrDI GCAATG_nn'

Cuts at:      0      67     966 
   Size:         67     899
 
BtsI GCAGTG_nn'

Cuts at:      0      45     966 
   Size:         45     921
 
EarI CTCTTCn'nnn_

Cuts at:      0      47     966 
   Size:         47     919
 
EcoRV GAT'ATC

Cuts at:      0     323     966 
   Size:        323     643
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     309     341     846     878     966 
   Size:        309      32     505      32      88

  Fragments arranged by size: 

                 505     309      88      32      32
 
HindIII A'AGCT_T

Cuts at:      0     518     966 
   Size:        518     448
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     575     966 
   Size:        575     391
 
MslI CAynn'nnrTG

Cuts at:      0     163     946     966 
   Size:        163     783      20

  Fragments arranged by size: 

                 783     163      20
 
MspA1I CmG'CkG

Cuts at:      0     126     966 
   Size:        126     840
 
NdeI CA'TA_TG

Cuts at:      0     396     966 
   Size:        396     570
 
NsiI A_TGCA'T

Cuts at:      0     340     463     966 
   Size:        340     123     503

  Fragments arranged by size: 

                 503     340     123
 
NspI r_CATG'y

Cuts at:      0      38     966 
   Size:         38     928
 
SfcI C'TryA_G

Cuts at:      0     906     966 
   Size:        906      60
 
StyI C'CwwG_G

Cuts at:      0     234     966 
   Size:        234     732
 
TatI w'GTAC_w

Cuts at:      0     224     604     966 
   Size:        224     380     362

  Fragments arranged by size: 

                 380     362     224
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      24      56     264     296     966 
   Size:         24      32     208      32     670

  Fragments arranged by size: 

                 670     208      32      32      24
 
XmnI GAAnn'nnTTC

Cuts at:      0     374     966 
   Size:        374     592
 

 Enzymes that do cut:

    AlwNI    BanII    BciVI     BclI    BfrBI    BsaBI    BsaXI    BseRI
     BsmI Bsp1286I    BspMI    BsrDI     BtsI     EarI    EcoRV    Hin4I
  HindIII     MmeI     MslI   MspA1I     NdeI     NsiI     NspI     SfcI
     StyI     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI