(Linear) (Six-base) MAPSORT of: KIEE6089.seq  Check: 9548  from: 1  to: 975



KIEE6089.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:23  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       7     975 
   Size:          7     968
 
AleI CACnn'nnGTG

Cuts at:      0     877     975 
   Size:        877      98
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      55     975 
   Size:         55     920
 
ApoI r'AATT_y

Cuts at:      0      70     962     975 
   Size:         70     892      13

  Fragments arranged by size: 

                 892      70      13
 
BanI G'GyrC_C

Cuts at:      0     934     975 
   Size:        934      41
 
BanII G_rGCy'C

Cuts at:      0     170     975 
   Size:        170     805
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      10     975 
   Size:         10     965
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     186     975 
   Size:        186     789
 
BclI T'GATC_A

Cuts at:      0     617     975 
   Size:        617     358
 
BfrBI ATG'CAT

Cuts at:      0      42     975 
   Size:         42     933
 
Bme1580I G_kGCm'C

Cuts at:      0     939     975 
   Size:        939      36
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     933     975 
   Size:        933      42
 
BsaAI yAC'GTr

Cuts at:      0     197     975 
   Size:        197     778
 
BsaBI GATnn'nnATC

Cuts at:      0     104     975 
   Size:        104     871
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     106     816     975 
   Size:        106     710     159

  Fragments arranged by size: 

                 710     159     106
 
Bsp1286I G_dGCh'C

Cuts at:      0     170     939     975 
   Size:        170     769      36

  Fragments arranged by size: 

                 769     170      36
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     282     975 
   Size:        282     693
 
Bsu36I CC'TnA_GG

Cuts at:      0     839     975 
   Size:        839     136
 
BtgI C'CryG_G

Cuts at:      0     389     975 
   Size:        389     586
 
DraIII CAC_nnn'GTG

Cuts at:      0     408     975 
   Size:        408     567
 
EarI CTCTTCn'nnn_

Cuts at:      0     226     544     975 
   Size:        226     318     431

  Fragments arranged by size: 

                 431     318     226
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       7     975 
   Size:          7     968
 
EcoRI G'AATT_C

Cuts at:      0      70     975 
   Size:         70     905
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     238     270     975 
   Size:        238      32     705

  Fragments arranged by size: 

                 705     238      32
 
MfeI C'AATT_G

Cuts at:      0     532     975 
   Size:        532     443
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     287     975 
   Size:        287     688
 
MslI CAynn'nnrTG

Cuts at:      0     175     337     877     975 
   Size:        175     162     540      98

  Fragments arranged by size: 

                 540     175     162      98
 
NcoI C'CATG_G

Cuts at:      0     389     975 
   Size:        389     586
 
NsiI A_TGCA'T

Cuts at:      0      44     975 
   Size:         44     931
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     408     975 
   Size:        408     567
 
SapI GCTCTTCn'nnn_

Cuts at:      0     544     975 
   Size:        544     431
 
SexAI A'CCwGG_T

Cuts at:      0     143     975 
   Size:        143     832
 
SfcI C'TryA_G

Cuts at:      0     906     975 
   Size:        906      69
 
SmlI C'TyrA_G

Cuts at:      0     912     975 
   Size:        912      63
 
SnaBI TAC'GTA

Cuts at:      0     197     975 
   Size:        197     778
 
SspI AAT'ATT

Cuts at:      0     291     498     975 
   Size:        291     207     477

  Fragments arranged by size: 

                 477     291     207
 
StyI C'CwwG_G

Cuts at:      0     389     975 
   Size:        389     586
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     900     975 
   Size:        900      75
 
TatI w'GTAC_w

Cuts at:      0     341     812     975 
   Size:        341     471     163

  Fragments arranged by size: 

                 471     341     163
 
XmnI GAAnn'nnTTC

Cuts at:      0      74     748     963     975 
   Size:         74     674     215      12

  Fragments arranged by size: 

                 674     215      74      12
 

 Enzymes that do cut:

     AcuI     AleI     AlfI     ApoI     BanI    BanII     BbsI    BciVI
     BclI    BfrBI Bme1580I    BpuEI    BsaAI    BsaBI    BseRI Bsp1286I
    BstXI   Bsu36I     BtgI   DraIII     EarI  Eco57MI    EcoRI    Hin4I
     MfeI     MmeI     MslI     NcoI     NsiI    PflMI     SapI    SexAI
     SfcI     SmlI    SnaBI     SspI     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MluI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SpeI     SphI     SrfI     StuI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI