Restriction map for
pFN21AE6086
(Linear) (Six-base) MAPSORT of: KIEE6086.seq Check: 8370 from: 1 to: 972
KIEE6086.orf
With 182 enzymes: SgfI *
December 5, 2014 13:23 ..
AccI GT'mk_AC
Cuts at: 0 178 217 972
Size: 178 39 755
Fragments arranged by size:
755 178 39
AflIII A'CryG_T
Cuts at: 0 721 972
Size: 721 251
BanII G_rGCy'C
Cuts at: 0 10 972
Size: 10 962
BmrI ACTGGGnnnn_n'
Cuts at: 0 538 972
Size: 538 434
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 716 972
Size: 226 490 256
Fragments arranged by size:
490 256 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 942 972
Size: 942 30
BsaAI yAC'GTr
Cuts at: 0 682 722 972
Size: 682 40 250
Fragments arranged by size:
682 250 40
BsaBI GATnn'nnATC
Cuts at: 0 640 972
Size: 640 332
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 199 229 972
Size: 199 30 743
Fragments arranged by size:
743 199 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 279 972
Size: 279 693
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 546 972
Size: 546 426
BsiHKAI G_wGCw'C
Cuts at: 0 195 972
Size: 195 777
Bsp1286I G_dGCh'C
Cuts at: 0 10 195 972
Size: 10 185 777
Fragments arranged by size:
777 185 10
BspHI T'CATG_A
Cuts at: 0 401 584 972
Size: 401 183 388
Fragments arranged by size:
401 388 183
BsrDI GCAATG_nn'
Cuts at: 0 821 972
Size: 821 151
BsrFI r'CCGG_y
Cuts at: 0 316 972
Size: 316 656
BssSI C'ACGA_G
Cuts at: 0 572 972
Size: 572 400
BstBI TT'CG_AA
Cuts at: 0 508 972
Size: 508 464
BstXI CCAn_nnnn'nTGG
Cuts at: 0 828 972
Size: 828 144
BstYI r'GATC_y
Cuts at: 0 482 972
Size: 482 490
BstZ17I GTA'TAC
Cuts at: 0 179 218 972
Size: 179 39 754
Fragments arranged by size:
754 179 39
Bsu36I CC'TnA_GG
Cuts at: 0 877 972
Size: 877 95
BtsI GCAGTG_nn'
Cuts at: 0 88 490 620 768 972
Size: 88 402 130 148 204
Fragments arranged by size:
402 204 148 130 88
EaeI y'GGCC_r
Cuts at: 0 368 972
Size: 368 604
EarI CTCTTCn'nnn_
Cuts at: 0 100 389 757 972
Size: 100 289 368 215
Fragments arranged by size:
368 289 215 100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 716 972
Size: 226 490 256
Fragments arranged by size:
490 256 226
EcoO109I rG'GnC_Cy
Cuts at: 0 953 972
Size: 953 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 566 598 972
Size: 29 537 32 374
Fragments arranged by size:
537 374 32 29
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 972
Size: 99 873
MscI TGG'CCA
Cuts at: 0 370 972
Size: 370 602
MslI CAynn'nnrTG
Cuts at: 0 139 172 583 646 972
Size: 139 33 411 63 326
Fragments arranged by size:
411 326 139 63 33
MspA1I CmG'CkG
Cuts at: 0 631 694 972
Size: 631 63 278
Fragments arranged by size:
631 278 63
NdeI CA'TA_TG
Cuts at: 0 373 972
Size: 373 599
PflMI CCAn_nnn'nTGG
Cuts at: 0 366 502 972
Size: 366 136 470
Fragments arranged by size:
470 366 136
PfoI T'CCnGG_A
Cuts at: 0 203 972
Size: 203 769
PmlI CAC'GTG
Cuts at: 0 722 972
Size: 722 250
PpuMI rG'GwC_Cy
Cuts at: 0 953 972
Size: 953 19
PstI C_TGCA'G
Cuts at: 0 296 972
Size: 296 676
PvuII CAG'CTG
Cuts at: 0 631 694 972
Size: 631 63 278
Fragments arranged by size:
631 278 63
SapI GCTCTTCn'nnn_
Cuts at: 0 100 757 972
Size: 100 657 215
Fragments arranged by size:
657 215 100
SexAI A'CCwGG_T
Cuts at: 0 125 972
Size: 125 847
SfcI C'TryA_G
Cuts at: 0 292 870 972
Size: 292 578 102
Fragments arranged by size:
578 292 102
SmlI C'TyrA_G
Cuts at: 0 957 972
Size: 957 15
SnaBI TAC'GTA
Cuts at: 0 682 972
Size: 682 290
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 450 482 972
Size: 450 32 490
Fragments arranged by size:
490 450 32
Enzymes that do cut:
AccI AflIII BanII BmrI BpmI BpuEI BsaAI BsaBI
BsaXI BseRI BsgI BsiHKAI Bsp1286I BspHI BsrDI BsrFI
BssSI BstBI BstXI BstYI BstZ17I Bsu36I BtsI EaeI
EarI Eco57MI EcoO109I Hin4I MmeI MscI MslI MspA1I
NdeI PflMI PfoI PmlI PpuMI PstI PvuII SapI
SexAI SfcI SmlI SnaBI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaI
BsaHI BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrGI BssHII BstAPI BstEII BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII TatI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI